| 巯基自组装的压电DNA传感器技术研究 |
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| 引用本文： | 高志贤,张清敏,姜永强,晁福寰,戴树桂. 巯基自组装的压电DNA传感器技术研究[J]. 南开大学学报(自然科学版), 2002, 35(3): 111-117 |
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| 作者姓名： | 高志贤 张清敏 姜永强 晁福寰 戴树桂 |
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| 作者单位： | [1]南开大学环境科学与工程学院，天津300071 [2]军事医学科学院，北京100850 |
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| 基金项目： | 国家自然科学基金 ( 39970 6 4 4)，天津市自然科学基金 ( 0 0 36 0 81 1 1 ) |
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| 摘 要： | 采用先将寡核苷酸分子标记上巯基基团，通过自组装技术将巯基修饰的寡核苷酸固定于金电极上，研制成的压电DNA传感器一致性，特异性都较好，每次检测杂交后，经过电极的再生，传感器可以重复使用3次，用该传感器检测和传统的斑点杂交方法相比较，具有快速，灵敏，简便等优越性。
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| 关 键 词： | 压电DNA传感器 葡萄球菌肠毒素B 巯基自组装 寡核苷酸 压电生物传感器 分子标记 |
| 文章编号： | 0465-7942(2002)03-0111-07 |
| 修稿时间： | 2001-11-18 |
THE RESEARCH OF THE THIOLATED SELF-ASSEMBLED MONOLAYERS TECHNOLOGY FOR PIEZOELECTRIC DNA SENSOR |
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| GAO Zhixian ,ZHANG Qingmin ,JIANG Yongqiang ,CHAO Fuhuan ,DAI Shugui. THE RESEARCH OF THE THIOLATED SELF-ASSEMBLED MONOLAYERS TECHNOLOGY FOR PIEZOELECTRIC DNA SENSOR[J]. Acta Scientiarum Naturalium University Nankaiensis, 2002, 35(3): 111-117 |
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| Authors: | GAO Zhixian ZHANG Qingmin JIANG Yongqiang CHAO Fuhuan DAI Shugui |
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| Affiliation: | GAO Zhixian 1,ZHANG Qingmin 1,JIANG Yongqiang 2,CHAO Fuhuan 2,DAI Shugui 1 |
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| Abstract: | Self-assembled monolayer technology has been applied in biosensor or chemical sensor. In the experiment, the piezoelectric DNA sensor was delveloped employing the thiolated oligonuleotide immobilized on gold electrode surface by self-assembled characteristic. The consistency and speciality of the sensor adopted above immobilization method was well. It can reuse three times or more than three times by electrode regeneration. The detection method is sensitive, rapid, simple and convenient comparing with dot hybridization method.$$$$ |
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| Keywords: | piezoelectric DNA sensor staphylococcal enterotoxin B thiolated molecular self-assembled monolayers |
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