黑芝麻下胚轴离体培养的研究

以晋芝3号黑芝麻(Sesamum indicumDC)无菌苗的下胚轴作为外植体,诱导培养基以MS为基本培养基,添加2.0mg/L6-BA与(0.1mg/L,0.2 mg/L,0.3 mg/L,0.4 mg/L,0.5 mg/L)以及NAA(0.5 mg/L,1.0 mg/L,1.5 mg/L,2.0 mg/L),共9种组合;生根培养基以1/2MS为基本培养基,添加NAA(0.1 mg/L,0.3 mg/L,0.5 mg/L)以及0.1 mg/LNAA和0.1 mg/LIAA,共4种组合,旨在此培养基上诱导黑芝麻下胚轴产生愈伤组织并分化出再生植株。结果表明:在MS+2.0 mg/L 6-BA+0.2 mg/L IAA培养基上的再生诱导率最高,可达81.8%,并且可使部分下胚轴切段形态学上端形成芽;在1/2MS+0.1mg/L NAA培养基上,再生苗的生根率达100%。

Study on in Vitro Culture of Hypocotyl of Black Sesame

The hypocotyl of aspectic seedings of the black sesame （Sesamum indicum DC, cv. Jinzhi No. 3 ） was used as the explants. The MS basic medium, supplemented with varous combinations of 6 - BA （2.0 mg/L）, IAA （0.1 rag/L, 0.2 mg/L, 0.3 mg/L, 0.4 mg/L or 0.5 mg/L） and NAA （0.5 mg/L, 1.0 rag/L, 1.5 mg/L or 2.0 mg/L） were used as the callus induction and regeneration media. The 1/2MS basic medium, added with varous combinations of NAA （0.1 rag/L, 0.3 rag/L, 0.5 rag/L）, NAA （0.1 mg/L） and IAA （0.1 mg/L） was used as the rooting media. We studied the callus induced from the hypocotyl and regenerated plants differentiated from the callus in the role of different media. The results showed that when inductive differentiation medium was MS ＋ 2.0 mg/L 6 - BA ＋ 0.2 mg/L IAA, the average induction rate was the highest. Some hypocotyl can cut off the morphological top to form buds. When the rooting medium was 1/2 MS ＋ 0.1 mg/L NAA, the rooting rate was 100%.