| Effect of New O-Superfamily Conotoxin on Voltage-Activated Currents of Hippocampal Neurons |
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| 作者姓名： | 李湛 何湘平 戴秋云 黄培堂 谢佐平 |
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| 作者单位： | [1]DepartmentofBiologicalSciencesandTechnology,TsinghuaUniversity,Beijing100084,China [2]InstituteofBiotechnology,Beijing100071,China |
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| 基金项目： | the National Ocean High-Tech Foundation of China (No. 819-06-04) |
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| 摘 要： | The effects of a new O-superfamily conotoxin, SO3, on sodium current (INa), transient A-type potassium currents (IA), and delayed rectified potassium currents (IK), were examined in cultured rat hippocampal neurons using the whole-cell patch clamp technique. Addition of SO3 caused a concentration-dependent, rapidly developing, and reversible inhibition of voltage-activated currents. The IC50 values for the blockage of INa, IA, and IK were calculated as 0.49, 33.9, and 7.6 μmol/L, respectively. The determined Hill coefficients were 1.7, 0.6, and 1.2, respectively. These results indicate that SO3 can selectively inhibit neuronal sodium and potassium currents.
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| 关 键 词： | 海马趾神经 钠电流 钾电流 SO3 痛觉缺失 |
Effect of New O-Superfamily Conotoxin on Voltage-Activated Currents of Hippocampal Neurons |
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| LI Zhan,HE Xiangping,DAI Qiuyun,HUANG Peitang ? XIE Zuoping.Effect of New O-Superfamily Conotoxin on Voltage-Activated Currents of Hippocampal Neurons[J].Tsinghua Science and Technology,2004,9(1):64-68. |
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| Authors: | LI Zhan HE Xiangping DAI Qiuyun HUANG Peitang ? XIE Zuoping |
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| Institution: | LI Zhan,HE Xiangping,DAI Qiuyun,HUANG Peitang ? XIE Zuoping ** Department of Biological Sciences and Technology,Tsinghua University,Beijing 100084,China, ?Institute of Biotechnology,Beijing 100071,China |
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| Abstract: | The effects of a new O-superfamily conotoxin, SO3, on sodium current (INa), transient A-type potassium currents (IA),and delayed rectified potassium currents (IK), were examined in cultured rat hippocampal neurons using the whole-cell patch clamp technique. Addition of SO3 caused a concentration-dependent, rapidly developing, and reversible inhibition of voltage-activated currents. The IC50 values for the blockage of INa , IA, and IK were calculated as 0.49, 33.9, and 7.6 mmol/L, respectively. The determined Hill coefficients were 1.7, 0.6, and 1.2, respectively. These results indicate that SO3 can selectively inhibit neuronal sodium and potassium currents. |
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| Keywords: | hippocampal neuron patch-clamp SO3 sodium current potassium current |
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