芽孢杆菌组合对海水养殖水体COD的降解效果

【目的】获得一种降解性能优于单菌株的菌株组合,更好地改善目前工业化密度养殖的水体水质。【方法】从刺参(Stichopus japonicus)养殖池塘分离降解效果良好的单菌株,根据菌株之间的拮抗效应,按照2株菌或3株菌进行组合,研究各组合菌株的降解效果。【结果】2株菌的组合第6天时COD含量的均值为422.57mg/L,标准差为63.85,3株菌的组合第6天时COD含量的均值为365.61mg/L,标准差为67.63,说明3株菌的组合整体降解效果比2株菌的组合好,且更容易获得降解效率高的组合,但并不是所有3株菌的组合降解效果都优于2株菌的组合。【结论】确定最优菌株组合为C14(菌株B2,菌株B6,菌株B11),COD降解率为64.29%。经鉴定,菌株B2为枯草芽孢杆菌(Bacillus subtilis),菌株B6为巨大芽孢杆菌(Bacillus megaterium),菌株B11为嗜碱性芽孢杆菌(Bacillus alcalophilus),为今后的实践应用提供参考。

Study on COD Degradation Capability of Bacillus Combinations

Objective]To improve the water quality in high stocking density of industrial aqua-culture,a kind of strain combination,with degradation capability better than single strain, was found.Methods]Several bacterial strains with good degradation property were separated from Stichopus japonicus culture ponds.Antagonistic effect between strains was studied.Ac-cording to the results of the antagonistic effect,the degradation capability of COD of the combinations of 2 or 3 strains were studied.Results]The mean COD of the combinations of two strains at the 6th day was 422.57 mg/mL,and standard deviation was 63.85.The mean COD of the combinations of three strains at the 6th day was 365.61 g/mL and the standard deviation was 67.63.The results showed that combinations of three strains compared to com-binations of two strains were easier to get a combination with better degradation efficiency.Conclusion]Finally,the optimal combination was C14(strain B2,strain B6,strain B11)with COD degradation rate of 64.29%.The strain B2,strain B6 and strain B11 were separately identified as Bacillus subtilis,Bacillus megaterium and Bacillus alcalophilus.The re-sult was a reference for future applications of strains.