| 野大麦耐盐变异体的筛选和植株再生 |
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| 引用本文： | 朱进.野大麦耐盐变异体的筛选和植株再生[J].南京师大学报,1998,21(1):82-85. |
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| 作者姓名： | 朱进 |
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| 作者单位： | 南京军区军事医学研究所，中国人民解放军农牧大学生物教研室 |
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| 摘 要： | 以野大麦的成熟胚为外植体诱国组织，选取淡黄色，易碎的胚佃组织为材料，进行细胞悬浮培养。
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| 关 键 词： | 野大麦 耐盐变异体 植株再生 筛选 |
Selection for Sodium Chloride Tolerant Variants of Wild Barley and Plant Regeneration |
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| Abstract: | Mature embryos of wild barley Horedum brevi subulatum (Trin.)Link were used as explants to induce callus.Yellowish and friable embryogenic calli were adopted to establish suspension cell cultures.After suspension cultures treated by ethylmethane sulfonate (EMS), sodium chloride tolerant variants were selected from them which cultured on saline medium.The results obtained from these studies showed that the variants could grow for a long time on 1.2% NaCl containing medium,while the controls died gradually.Free L-proline content of 1.2% NaCl tolerant variants was as much as 6.68 times of that of the control,and relative electrical conductivity ratio was 29.9% less than that of the control.Their esterose isozyme patterns were also different from the control with two additional bands,while their peroxidase isozyme bands were similar.These analyses showed that NaCl-selected calli had the characters of NaCl-tolerance. |
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| Keywords: | wild barley mature embryo NaCl-tolerant variants plant regeneration |
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