| 产丁醇重组大肠杆菌的构建及初步发酵 |
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| 引用本文： | 张秋妍,郭亭,杜腾飞,汤艳,贺爱永,姜岷. 产丁醇重组大肠杆菌的构建及初步发酵[J]. 南京工业大学学报(自然科学版), 2012, 34(4): 118-122 |
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| 作者姓名： | 张秋妍 郭亭 杜腾飞 汤艳 贺爱永 姜岷 |
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| 作者单位： | 1. 南京工业大学生物与制药工程学院,江苏南京,210009
2. 南京工业大学生物与制药工程学院,江苏南京210009;广州甘蔗糖业研究所,广东广州510316 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目，林业公益性专项基金资助项目，材料化学工程国家重点实验室基金资助项目，江苏省“青蓝工程”资助项目 |
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| 摘 要： | 将丙酮丁醇梭菌ATCC 824丁醇合成途径中分别编码硫解酶(THL)、3-羟基丁酰-CoA脱氢酶(HBD)、巴豆酸酶(CRT)、丁酰-CoA脱氢酶(BCD)和醛/醇脱氢酶(BDH)的关键基因thil、hbd、crt、bcd-etfB-etfA、adhE在大肠杆菌中进行重组表达,构建出3株产丁醇的重组大肠杆菌JM109-99d、DL1346 99d和AFP111-99d。选取摇瓶发酵产丁醇最高(0.52 g/L)的重组大肠杆菌DL1346-99d进行上罐发酵,丁醇产量可达1.07g/L。
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| 关 键 词： | 丁醇 大肠杆菌 发酵 丙酮丁醇梭菌 |
Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli for butanol production |
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| ZHANG Qiuyan , GUO Ting , DU Tengfei , TANG Yan , HE Aiyong , JIANG Min. Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli for butanol production[J]. Journal of Nanjing University of Technology, 2012, 34(4): 118-122 |
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| Authors: | ZHANG Qiuyan GUO Ting DU Tengfei TANG Yan HE Aiyong JIANG Min |
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| Affiliation: | 1 (1.College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China; 2.Guangzhou Sugarcane Industry Research Institute,Guangzhou 5103 16,China) |
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| Abstract: | A recombinant butanol pathway composed of C.acetobutylicum ATCC 824 genes,thil,hbd,crt, bcd-etfB-etf A,and adhE coding for acetyl-CoA acetyltransferase(THL),β-hydroxybutyryl-CoA dehydrogenase (HBD),3-hydroxybutyryl-CoA dehydratase(CRT),butyryl-CoA dehydrogenase(BCD),and butanol dehydrogenase(BDH)was constructed under the trc promoter control and was transferred to E.coli JM109-99d,DL1346-99d,and AFP111-99d.Results of fermentation in sealed bottles showed that the recombinant E.coli DL1346-99d yielded the highest butanol concentration of 0.52 g/L,and the yield of butanol was up to 1.07 g/L. |
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| Keywords: | butanol Escherichia coli fermentation Clostridium acetobutylicum |
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