| 红碱淖遗鸥血液基因组DNA提取方法的比较研究 |
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| 引用本文： | 行文珍,雷 忻,戚珍珠,等.红碱淖遗鸥血液基因组DNA提取方法的比较研究[J].延安大学学报(自然科学版),2014(1):58-60,69. |
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| 作者姓名： | 行文珍 雷 忻 戚珍珠 等 |
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| 作者单位： | 延安大学生命科学学院,陕西延安716000 |
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| 基金项目： | 国家自然科学基金项目（31240076）;国家大学生科技创新项目（201210719020）;陕西省自然科学基础研究计划项目（2012JM3012）;陕西省高水平大学建设专项资金资助项目（2012SXTS03）;陕西省教育厅科学研究项目（2013JK0710）;延安市科学技术研究发展计划项目（2012KS-15）;延安大学博士科研启动项目（YD2007-112） |
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| 摘 要： | 采用酚-氯仿法和改良Miller盐析法从红碱淖遗鸥冷冻血液样品中提取基因组DNA,以筛选遗鸥冷冻血液DNA的最佳提取方法.结果显示：酚-氯仿法琼脂糖凝胶电泳DNA样品亮度和清晰度较高,条带较粗,纯度较高;而改良Miller盐析法提取基因组DNA,获得率较低,并且难以去除杂质;酚-氯仿法提取时,酶解16 h比酶解10 h的DNA提取效果好,说明在一定时间范围内,延长酶解时间可有效地提高DNA提取效果.上述结果提示,酚-氯仿法对遗鸥冷冻血液基因组DNA提取的效果优于改良Miller盐析法.
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| 关 键 词： | 遗鸥 DNA提取 酚-氯仿法 改良Miller盐析法 |
Comparative Studies on the Methods of DNA Extraction from Blood of Ichthyaetus relictus in Hongjiannao |
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| XING Wen-zhen,LEI Xin,QI Zhen-zhu,TIAN Peng-fei,ZHANG Jing-jing.Comparative Studies on the Methods of DNA Extraction from Blood of Ichthyaetus relictus in Hongjiannao[J].Journal of Yan'an University(Natural Science Edition),2014(1):58-60,69. |
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| Authors: | XING Wen-zhen LEI Xin QI Zhen-zhu TIAN Peng-fei ZHANG Jing-jing |
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| Institution: | ( College of Life Science, Yanan University, Yanan 716000, China) |
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| Abstract: | In order to find a better method for genome DNA extraction from frozen blood of Ichthyaetus relictus in Hongjiannao, the DNA extraction were comparative studied using both the phenol- chloroform method and the modified Miller salting - out method. Comparing the DNA agarose gel electrophoretogram made by two methods, the DNA extracted by phenol -chloroform method were lighter, clearer and purer than that by the modified Miller sal- ting-out method. Moreover, compared enzymolysis for 10 h with for 16 h by phenol- chloroform method, the effects of DNA extracted for 16 h were better than that for 10 h. The results indicated that the phenol - chloroform method was suitable for genome DNA extraction from frozen blood of I. relictus. |
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| Keywords: | Ichthyaetus relictus DNA extraction phenol -chloroform method modified Miller salting -out method |
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