Cloning and sequence analysis of Sox genes in a tetraploid cyprinid fish, Tor douronensis |
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Authors: | BaoCheng Guo JunBing Li ChaoBo Tong ShunPing He |
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Institution: | (1) Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China;(2) Graduate University of the Chinese Academy of Sciences, Beijing, 100049, China |
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Abstract: | A PCR survey for Sox genes in a young tetraploid fish Tor douronensis (Teleostei: Cyprinidae) was performed to access the evolutionary fates of important functional genes after genome duplication
caused by polyploidization event. Totally 13 Sox genes were obtained in Tor douronensis, which represent SoxB, SoxC and SoxE groups. Phylogenetic analysis of Sox genes in Tor douronensis provided evidence for fish-specific genome duplication, and suggested that Sox19 might be a teleost specific Sox gene member. Sequence analysis revealed most of the nucleotide substitutions between duplicated copies of Sox genes caused by tetraploidization event or their orthologues in other species are silent substitutions. It would appear that
the sequences are under purifying selective pressure, strongly suggesting that they represent functional genes and supporting
selection against all null allele at either of two duplicated loci of Sox4a, Sox9a and Sox9b. Surprising variations of the intron length and similarities of two duplicated copies of Sox9a and Sox9b, suggest that Tor douronensis might be an allotetraploidy.
Supperted by the National Natural Science Foundation of China (Grant No. 30530120) |
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Keywords: | Tor douronensis Sox gene tetraploidy genome duplication gene diversity |
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