| AtGT-3b基因克隆及原核表达与纯化 |
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| 引用本文： | 李秋莉,陈丽娜,关秋玲,于连. AtGT-3b基因克隆及原核表达与纯化[J]. 辽宁师范大学学报(自然科学版), 2012, 35(3): 377-382 |
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| 作者姓名： | 李秋莉 陈丽娜 关秋玲 于连 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连,116081 |
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| 基金项目： | 国家自然科学基金项目(30871389);辽宁省教育厅重点实验室项目(LS2010093) |
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| 摘 要： | GT-3b转录因子是一个受NaCl和病原体诱导表达的GT-1-like转录因子,它能与GT-1 cis-element( GAAAAA)相互作用,促进下游基因的表达,在植物耐盐中起着重要的调节作用.通过分离了拟南芥(Arabidopsis thaliana)AtGT-3b基因,克隆到原核表达载体pCold TF中,并在大肠杆菌(Escherichia coli)BL21中进行融合表达;通过纯化得到AtGT-3b融合蛋白,以期用于研究其与GT-1顺式作用元件在体外的相互作用.
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| 关 键 词： | AtGT-3b基因 原核表达 蛋白纯化 |
Cloning,prokaryotic expression and purification of AtGT-3b gene |
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| LI Qiu-li , CHEN Li-na , GUAN Qiu-ling , YU Lian. Cloning,prokaryotic expression and purification of AtGT-3b gene[J]. Journal of Liaoning Normal University(Natural Science Edition), 2012, 35(3): 377-382 |
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| Authors: | LI Qiu-li CHEN Li-na GUAN Qiu-ling YU Lian |
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| Affiliation: | (College of Life Science,Liaoning Normal University,Dalian 116081,China) |
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| Abstract: | GT-3b is a GT-l-like transcription factor, induced by NaCl and pathogen. It can interact with GT-1 cis-element （GAAAAA） and promot the expression of its downstream genes. It plays an important role in plant salt tolerance. In this study, AtGT-3b gene was isolated from Arabidopsis thaliana, and cloned into expression vector pCold TF. The AtGT-3b fusion protein were expressed in E. coli BL21, and purified by Ni-Chelating chromatography. The purified AtGT-3b fusion protein can be used in studying the in vitro ：interaction with GT-1 cis-acting element. |
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| Keywords: | AtGT-3b gene prokaryotic expression protein purification |
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