无机离子对嗜盐放线菌株YIM 90615代谢产物多样性的影响

为了研究NaCl浓度、金属离子(Cr7+、Mg2+及K+)、氯化钪及卤素(I-、Br-及F-)对嗜盐放线菌株YIM 90615液体发酵过程中次级代谢产物多样性的影响,作者采用HPTLC(high performance thin layer chromatography)技术,结合紫外(254 nm)检测、碘及硫酸-茴香醛显色方法,对该菌代谢产物的HPTLC图谱进行了定性分析.结果显示,NaCl添加质量浓度为100 g/L时该菌株发酵效果最佳,有利于其代谢产物的多样性及量的积累;Cr7+和K+有利于其次生代谢产物的产生;1 g/L的氯化钪能增加其次生代谢产物的丰富性;F-对其次生代谢产物多样性有较好的促进作用.试验结果表明外源添加一定浓度和种类的盐离子有助于提升YIM 90615液体发酵过程中次生代谢产物多样性,为新型抗生素及其它有用化合物的产生创造合适条件,同时有助于嗜盐放线菌发酵培养基的筛选工作.

The influences of inorganic ions on the diversities of secondary metabolitesproduced by Actinopolyspora YIM 90615

To understand the effect of salt irons,such as NaCl, metal irons (Cr⁷⁺,Mg²⁺,and K^+),ScCl₃,and halogen (I^-  Br^- , and F^- ),on the diversity of secondary metabolites produced by Actinopolyspora YIM 90615,HPTLC chromatographed by UV254,iodine and anisaldehyde-sulfuric acid were performed.The results showed that application of NaCl at the final concentration of 100g/L showed the best promotion to the growth of the strain and the diversity of secondary metabolites production,and Cr⁷⁺,K⁺ also help to produce more secondary metabolites,additional 1g/L ScCl₃ and F^- could promote the diversity of secondary metabolites too.These results suggested that exogenous application of salt irons could obviously stimulate the growth of strain YIM 90615 and the productivity and diversity of second metabolisms,which could provide the theoretical basis for the practical application of Actinopolyspora in future and create suitable conditions for producing new antibiotics and other useful compounds.It also provides theoretical basis for the selection of the fermentation medium for other Actinomycetes.