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食品体外抗氧化能力评价方法探讨
引用本文:张迪,籍保平,周峰,吴薇.食品体外抗氧化能力评价方法探讨[J].北京工商大学学报(自然科学版),2012,30(1):20-25.
作者姓名:张迪  籍保平  周峰  吴薇
作者单位:1. 中国农业大学食品科学与营养工程学院,北京,100083
2. 中国农业大学工学院,北京,100083
基金项目:国家自然科学基金面上项目(31171647);国家十二五科技支撑计划项目(2011BAD08B03-01)
摘    要:食品抗氧化能力评价是功能性食品研究的重要领域.目前体外抗氧化能力评价存在着定义不清,实验方法混乱等方面的问题,极大地阻碍了人们比较和筛选抗氧化功能性食品.因此,开发一种能准确评价样品抗氧化能力的方法是亟待解决的世界性难题.从诱导方式,反应机理,结果指标等角度讨论主要体外化学抗氧化评价方法和体外细胞抗氧化评价方法的特点以及他们的缺陷,并对体外抗氧化评价方法的发展与创新提出了展望.

关 键 词:氧化应激  自由基  电化学  细胞模型

Advances in Antioxidant Activity Assessment Assays for Food
ZHANG Di,JI Bao-Ping,ZHOU Feng and WU Wei.Advances in Antioxidant Activity Assessment Assays for Food[J].Journal of Beijing Technology and Business University:Natural Science Edition,2012,30(1):20-25.
Authors:ZHANG Di  JI Bao-Ping  ZHOU Feng and WU Wei
Institution:1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China; 2.College of Engineering,China Agricultural University,Beijing 100083,China)
Abstract:The assessment of antioxidant activity in vitro is an important area in functional food research.However,there were many problems,such as the confusing specification of antioxidant capacity and the improper application of antioxidant activity assessment assays,which hampered the comparison and selection of antioxidant functional foods greatly.Thus,development of a validated method for antioxidant activity assessment is a worldwide problem.In this study,the characteristics and shortcomings of the antioxidant activity assessment methods(the chemical and cellular assays) were discussed in terms of inducing method,reaction mechanisms,and result indicators.In addition,the development prospects of the antioxidant activity assessment methods were proposed.
Keywords:oxidative stress  free radicals  electrochemistry  cell model
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