| 厦门霉素生物合成基因簇在白色链霉菌及吸水链霉菌FR-008中的异源表达 |
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| 引用本文： | 张金龙.厦门霉素生物合成基因簇在白色链霉菌及吸水链霉菌FR-008中的异源表达[J].信阳师范学院学报(自然科学版),2015(2):168-172. |
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| 作者姓名： | 张金龙 |
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| 作者单位： | 上海交通大学生命科学技术学院 |
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| 基金项目： | 国家自然科学基金项目(31400213) |
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| 摘 要： | 将携带有厦门霉素生物合成的整合质粒p LMO09404,通过供体菌大肠杆菌ET12567(p UZ8002)与受体菌白色链霉菌及吸水链霉菌FR-008进行接合转移,将得到的接合转移菌株经PCR验证后进行发酵,发酵液经HPLC和质谱检测.结果显示,厦门链霉菌来源厦门霉素基因簇可以在白色链霉菌及吸水链霉菌FR-008中异源表达.
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| 关 键 词： | 厦门霉素 白色链霉菌 吸水链霉菌FR-008 异源表达 |
Heterologous Expression of Benzopyran Biosynthesis Gene Cluster in Streptomyces albus and Streptomyces hygroscopicus FR-008 |
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| Zhang Jinlong.Heterologous Expression of Benzopyran Biosynthesis Gene Cluster in Streptomyces albus and Streptomyces hygroscopicus FR-008[J].Journal of Xinyang Teachers College(Natural Science Edition),2015(2):168-172. |
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| Authors: | Zhang Jinlong |
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| Institution: | Zhang Jinlong;School of Life Sciences and Biotechnology,Shanghai Jiao tong University; |
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| Abstract: | The plasmid p LMO09404 that carries xiamenmycin biosynthesis gene cluster was introduced into Streptomyces albus and Streptomyces hygroscopicus FR-008 from donor E. coli ET12567( p UZ8002). The integrated strains detected by PCR were inoculated into fermentation medium and the fermentation liquid was detected by HPLC and mass spectrometry. The results showed that Xiamenmycin has been heterologously expressed in Streptomyces albus and Streptomyces hygroscopicus FR-008. |
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| Keywords: | Xiamenmycin Streptomyces albus Streptomyces hygroscopicus FR-008 heterologous expression |
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