| 在杂合启动子控制下乙肝病毒表面抗原SA-28融合基因在酵母中的表达及调控 |
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| 引用本文： | 刘 婷,袁汉英,沈其梅,李育阳,汪垣.在杂合启动子控制下乙肝病毒表面抗原SA-28融合基因在酵母中的表达及调控[J].复旦学报(自然科学版),1998(4). |
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| 作者姓名： | 刘 婷 袁汉英 沈其梅 李育阳 汪垣 |
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| 作者单位： | 复旦大学(刘 婷，袁汉英，沈其梅，李育阳)，中国科学院上海生物化学研究所(汪垣) |
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| 基金项目： | 国家科委八六三高科技项目 |
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| 摘 要： | 采用酵母杂合启动子PADH2－CUP1或PADH2－GAPDH及终止子TADH1，构建了一系列酵母表达载体．在这些表达载体中插入乙肝病毒表面抗原S－preS1融合基因SA－28后，将合乙肝病毒表面抗原的表达单元克隆至高稳定质粒PHC11的BamHI位点．然后将表达质粒分别转化酿酒酵母Y16，Y19．对SA－28基因表达的研究表明，在酵母菌胞内实现了SA－28基因的高表达，且表达受葡萄糖浓度调控．
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| 关 键 词： | 酵母 杂合启动子 乙肝病毒表面抗原 |
Expression and Regulation of HBsAg Modified Gene SA-28 Driven by Hybrid Promoters in Yeast |
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| Liu Yiting, Yuan Hanying, Shen Qimei, Li Yuyang, Wang Yuan.Expression and Regulation of HBsAg Modified Gene SA-28 Driven by Hybrid Promoters in Yeast[J].Journal of Fudan University(Natural Science),1998(4). |
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| Authors: | Liu Yiting Yuan Hanying Shen Qimei Li Yuyang Wang Yuan |
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| Abstract: | Series of expression vectors were constructed, using hybrid promoters, PADH2-CUP1 or PADH2-GAPDH, and terminator TADH1. A modified antigen gene SA-28, coding for hepatitis B surface antigen (HBsAg) and preS1 epitope had been placed at down stream of these two hybrid promoters. After that, the expression cassettes were cloned into the BamH I site of a highly stable plasmid PHC11 to construct expression plasmids. Then they were transformed into two yeast strains, Y16 and Y19. A high level expression of S-preS1 was observed intracellularly, and the expression was regulated by the glucose concentration of the medium. |
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| Keywords: | yeast hybrid promoter HBsAg |
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