| 靶向多肽功能化阳离子聚合物携载ZNF580质粒对内皮细胞增殖的影响 |
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| 引用本文： | 朵兴红,姜萧韩,周若琦,冯亚凯,刘晶.靶向多肽功能化阳离子聚合物携载ZNF580质粒对内皮细胞增殖的影响[J].华南师范大学学报(自然科学版),2020,52(3):62-69. |
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| 作者姓名： | 朵兴红 姜萧韩 周若琦 冯亚凯 刘晶 |
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| 作者单位： | 1.青海民族大学化学化工学院，西宁 810007 |
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| 基金项目： | 青海民族大学高层次人才项目;青海省自然科学基金;教育部春晖计划;国家自然科学基金;青海民族大学校级本硕博(学生)项目 |
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| 摘 要： | 通过原子转移自由基聚合(ATRP)方法制备了以多面体低聚倍半硅氧烷(POSS)为骨架的POSS-(PDMAEMA)8(PPD)星型阳离子聚合物，通过巯基-双键化学反应，将特异性靶向EA.hy926内皮细胞的CREDVW多肽键接在PPD阳离子聚合物末端，得到POSS-(PDMAEMA)8-PPEGMA-CREDVW(PPD-CREDVW)阳离子聚合物.在n(N)/n(P)=5时，PPD-CREDVW阳离子聚合物携载pEGFP-ZNF580(pDNA)质粒自组装形成粒径为125.67±3.31 nm、zeta电位为10.64±1.65 mV的PPD-CREDVW/pDNA基因复合胶束.细胞实验结果显示:与PPD/pDNA基因复合胶束相比，PPD-CREDVW/pDNA基因复合胶束携载基因能力强、细胞毒性低、易被细胞摄取，能够显著提高EA.hy926内皮细胞的转染，促进细胞的增殖.
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| 关 键 词： | 多面体低聚倍半硅氧烷(POSS) REDV多肽 EA.hy926内皮细胞 增殖 |
| 收稿时间： | 2019-07-30 |
The Effect of Targeted Polypeptide Functionalized Cationic Polymer Loaded with ZNF580 Plasmid on Proliferation of Endothelial Cells |
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| Institution: | 1.School of Chemistry and Chemical Engineering, Qinghai University for Nationalities, Xining 810007, China2.School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China3.College of Eco-environment and Resources, Qinghai University for Nationalities, Xining 810007, China4.Key Laboratory of Resource Chemistry and Eco-environmental Protection in Tibetan Plateau, State Ethnic Affairs Commission, Xining 810007, China |
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| Abstract: | A star-shaped cationic polymer POSS-(PDMAEMA)8 (PPD) based on oligomeric sesquioxane (POSS) was prepared with atom transfer radical polymerization(ATRP). In order to prepare POSS-(PDMAEMA)8-PPEGMA-CREDVW (PPD-CREDVW) cationic polymer, the CREDVW peptide, which targets endothelial cells (EA.hy926), was grafted onto the end of PPD cationic polymer through the thiol-double bond click chemical reaction. When n(N)/n(P)=5, PPD-CREDVW cationic polymer loaded with pEGFP-ZNF580 (pDNA) plasmid could form PPD-CREDVW/pDNA gene composite micelle with a particle size of 125.6±3.31 nm and a zeta potential of 10.64±1.65 mV through self-assembly. Compared with PPD/pDNA gene complexes micelles, PPD-CREDVW/pDNA gene complexes micelles could be uptaken by cells, had strong capacity for gene-carrying and had low cytotoxicity. Thus it can significantly promote the transfection and proliferation of endothelial cells. |
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