| 高效液相色谱测定鸟氨酸脱羧酶活性方法的改进 |
| |
| 引用本文： | 魏凯,李恩中,李超坤,许晓军,宁黔冀.高效液相色谱测定鸟氨酸脱羧酶活性方法的改进[J].河南师范大学学报(自然科学版),2004,32(4):138-140. |
| |
| 作者姓名： | 魏凯 李恩中 李超坤 许晓军 宁黔冀 |
| |
| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007;河南师范大学,生命科学学院,河南,新乡,453007;河南师范大学,生命科学学院,河南,新乡,453007;河南师范大学,生命科学学院,河南,新乡,453007;河南师范大学,生命科学学院,河南,新乡,453007 |
| |
| 基金项目： | 河南省自然科学基金(20011800009)，河南省高校青年骨干教师资助计划资助项目 |
| |
| 摘 要： | 对柴彪新等报道的高效液相色谱测定鸟氨酸脱羧酶(ODC)活性的方法作了改进.结果表明,改进后的方法更灵敏,更简便,线性范围明显扩大,相关系数为0.9998,显著高于柴彪新等报道的0.9939.同时,新方法还可使苯甲酸和苯甲酰氯降到很低.
|
| 关 键 词： | 高效液相色谱(HPLC) 方法改进 鸟氨酸脱羧酶 腐胺 |
| 文章编号： | 1000-2367(2004)04-0138-03 |
| 修稿时间： | 2004年5月23日 |
Improvement for the high-performance liquid chromatography assay for the activity of ornithine decarboxylase |
| |
| WEI Kei,LI En-zhong,LI Chao-kun,XU Xiao-jun,NING Qian-ji.Improvement for the high-performance liquid chromatography assay for the activity of ornithine decarboxylase[J].Journal of Henan Normal University(Natural Science),2004,32(4):138-140. |
| |
| Authors: | WEI Kei LI En-zhong LI Chao-kun XU Xiao-jun NING Qian-ji |
| |
| Abstract: | The HPLC method reported by Chai Biao-xin et al for determination of the activity of ornithine decarboxylase was improved. Results indicated that the promoted method was more sensitive and simply for ODC assay. The linear rang was apparently extended and the correlation coefficient was up to 0.9998 which was higher than Chai Biao-xin's 0.9939. The ODC activity in 10 partially hepatectomized rats liver was mensurated by using this promoted method, and it was significantly higher than that of control group. |
| |
| Keywords: | high-performance liquid chromatography(HPLC) improvement ornithine decarboxylase(ODC) putrescine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
