| 小鼠骨髓造血基质细胞体外培养及其功能的研究 |
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| 引用本文： | 刘存仁,于善清,贺福初,吴祖泽.小鼠骨髓造血基质细胞体外培养及其功能的研究[J].山东大学学报(理学版),2002,37(2):172-175. |
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| 作者姓名： | 刘存仁 于善清 贺福初 吴祖泽 |
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| 作者单位： | 1. 山东大学生命科学学院,山东,济南,250100
2. 北京放射医学研究所,北京,100850 |
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| 摘 要： | 采用细胞培养从正常LACA雄性小鼠骨髓中分离得到1株造血基质细胞（HSC），该细胞胞体较大，呈多角形，不吞噬酵母多糖，HSC粘壁层有其培养上清液均具有明显刺激CFU－GM体外增殖分化的功能，将HSC输注到经射线照射的雌性小鼠体内，PCR技术检测结果显示，HSC具有重建受体小鼠造血微环境的作用。
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| 关 键 词： | 小鼠 骨髓 造血基质细胞 体外培养 功能 细胞培养 增殖分化 造血环境 |
| 文章编号： | 0559-7234(2002)02-0172-04 |
| 修稿时间： | 2000年11月30 |
STUDIES ON IN VITRO CULTURE AND FUNCTION OF HEMATO POIETIC STROMAL CELL FROM MURINE BONE MARROW |
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| LIU Cun ren ,YU Shan qing ,HE Fu chu ,WU Zu ze.STUDIES ON IN VITRO CULTURE AND FUNCTION OF HEMATO POIETIC STROMAL CELL FROM MURINE BONE MARROW[J].Journal of Shandong University,2002,37(2):172-175. |
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| Authors: | LIU Cun ren YU Shan qing HE Fu chu WU Zu ze |
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| Institution: | LIU Cun ren 1,YU Shan qing 1,HE Fu chu 2,WU Zu ze 2 |
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| Abstract: | A strain of hematopoietic stromal cell (HSC)was established from bone marrow in LACA male mouse by using cell culture technique.HSC was found to be large polygonal cells and not to phagocytose zymosan. The conditioned medium and adherent layer of HSC were capable of supporting the proliferation and differentiation of CFU-GM.It was also found that HSC was able to reestablish the hematopoietic microenvironment of murine bone marrow following intravenous injection. |
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| Keywords: | bone marrow hematopoietic stromal cell in vitro culture function |
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