| 流动注射化学发光测定抗坏血酸及其机理探讨 |
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| 引用本文： | 罗万芬,曾仁权.流动注射化学发光测定抗坏血酸及其机理探讨[J].河南师范大学学报(自然科学版),2004,32(1):69-72. |
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| 作者姓名： | 罗万芬 曾仁权 |
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| 作者单位： | 西南农业大学荣昌校区,基础部,重庆,402460;西南农业大学荣昌校区,基础部,重庆,402460 |
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| 摘 要： | 研究发现,N-溴代琥珀酰亚胺(NBS)在碱性介质条件下,可以氧化抗坏血酸,在荧光素的增敏作用下,产生较强的化学发光.基于此建立了测定痕量抗坏血酸的流动注射化学发光新方法.抗坏血酸在0.05～100μg/mL的浓度范围内与化学发光强度呈良好的线性关系,检测限为(3σ)8.7ng/mL,对0.5μg/mL的抗坏血酸连续测定11次,相对标准偏差为2.3%.将本方法用于抗坏血酸制剂和片剂含量的分析,并与药典标准方法对照,其结果比较一致,说明该方法准确、可靠.
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| 关 键 词： | 流动注射分析 化学发光 抗坏血酸 N-溴代琥珀酰亚胺 荧光素 |
| 文章编号： | 1000-2367(2004)01-0069-04 |
| 修稿时间： | 2003年9月20日 |
Flow injection chemiluminescence analysis of ascorbic acid |
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| LUO Wan-fen,ZENG Ren-quan.Flow injection chemiluminescence analysis of ascorbic acid[J].Journal of Henan Normal University(Natural Science),2004,32(1):69-72. |
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| Authors: | LUO Wan-fen ZENG Ren-quan |
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| Abstract: | A new method for the determination of ascorbic acid with flow injection chemiluminescence was described. It is based on the chemiluminescence emitted during the oxidation of ascorbic acid by N-bromosuccinimide (NBS) in alkaline medium in the presence of fluorescein as energy transfer. This method is sensitive, simple, rapid and reproductively. The linear range of determination for ascorbic acid was 0.05～100 μg/mL with the relative standard deviation of 2.3% (C=0.5 μg/mL, n=11). The detection limit was 8.7 ng/mL(3σ). The results obtained for the assay of commercial pharmaceutical preparations compared well with those obtained by the standard methods and demonstrated good accuracy and precision.The recommended method has been successfully used for analysis of commercial formulation with satisfactory results. |
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| Keywords: | chemiluminescence flow-injection analysis ascorbic acid N-bromosuccinimide (NBS) fluorescein |
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