| HPLC法测定蒙药哈斯-哈图古日-15中没食子酸的含量 |
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| 引用本文： | 周风琴,赵玉英,张良,姚立伟,王海燕.HPLC法测定蒙药哈斯-哈图古日-15中没食子酸的含量[J].内蒙古民族大学学报(自然科学版),2014(3):277-279. |
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| 作者姓名： | 周风琴 赵玉英 张良 姚立伟 王海燕 |
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| 作者单位： | [1] 内蒙古民族大学化学化工学院, 内蒙古通辽028043 [2] 天然产物开发与废弃物利用研究所 ,内蒙古通辽028043 |
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| 基金项目： | 国家自然科学基金资助项目（21261015） |
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| 摘 要： | 建立用HPLC法测定蒙药哈斯-哈图古日-15中没食子酸的方法.色谱柱为：Hypcrsil C18 ODS（4.6mmX250mm,5μL）,流动相为乙腈-水-磷酸（体积比为5：95：0.02），流速为0.8 mL·min-1,检测波长为273nm，柱温为30℃.没食子酸在（0.40～1.2）mg·mL-1范围内线性关系良好（y=9E＋06x-127588，r=0.9999），平均回收率94.95%（RSD=2.24%）.该方法可作为蒙药及蒙药材的没食子酸的含量测定方法.
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| 关 键 词： | 哈斯-哈图古日-15 没食子酸 HPLC |
Determination the Content of Gallic Acid in Haas-Hatuguri-15 by HPLC |
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| ZHOU Feng-qin,ZHAO Yu-ying,ZHANG Liang,YAO Li-wei,WANG Hai-yan.Determination the Content of Gallic Acid in Haas-Hatuguri-15 by HPLC[J].Journal of Inner Mongolia University for the Nationalities(Natural Sciences),2014(3):277-279. |
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| Authors: | ZHOU Feng-qin ZHAO Yu-ying ZHANG Liang YAO Li-wei WANG Hai-yan |
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| Institution: | 1.College of Chemistry and Engineering, Inner Mongolia University for Nationalities, Tongliao 028043, China; 2.Center of Analysis and Testing of Development of Natural Productsand Waste Utilization Research Institute, Tongliao 028043, China) |
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| Abstract: | To establish a HPLC method for determination the content of Gallic Acid in Haas-Hatuguri-15. The high performance liquid chromatography（HPLC）separation was performend on Hypcrsil C18 ODS（4.6mmX250mm,5μL）. The mobile phase consisted of acetonitrile-water-phosphate （The volume ratio of 5：95：0.02 ）. The flow rate was 0.8mL·min-1 , the detection wavelength was 273nm, the column temperation was 30℃. Gallic Acid showed a good lin-ear relationship（y=9E＋06x-127588,r=0.9999）in the range of 0.40-1.20mg·mL-1 , the average recovery rate was 94.95%, RSD=2.24%. This method can be used as the Gallic Acid in Mongolian medicine and Mongolian medicine de-termination method. |
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| Keywords: | HPLC HPLC Gallic Acid |
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