冷冻保存对精子运动的影响

目的：观察精子运动状态,其中包括精子折断现象与冷冻损伤的关系。方法：对精液标本进行一步冷冻保存和分步冷冻保存,并用鉴别精子形态湿片法对冷冻前后精子运动状态及折断率进行评价。结果：冷冻保存复温后的精子活动率与冷冻前的精了活动率比较明显下降（P＜0．01）;含冷冻保护剂的实验组与不含冷冻保护剂的对照组比较,精子率呈显著性差异（P＜0．01）,精子折断率无显著性差异（P＞0．05）;一步冷冻和分步冷冻保存的精子之间活动率及折断率相比较,无显著性差异（P＞0．05）。结论：冷冻保存易造成精子活动率下降、运动形态异常,但不能显著地导致精子折断发生率增加。

Effect of cryopreservation on human sperm motility

Aim:To investigate changes on sperm motility after freezing-thawing and the relationship between sperm fracture and cryopreservation. Methods: Human semen samples were cryopreserved by one-step method as well as multiple-step method. Sperm morphology wet preparation was used to eva- luate sperm motility and the sperm fracture rate. Results: Post-thawing sperm motility was significantly lower than that before (P<0 01), whereas difference between sperm fracture rates was not seen (P>0 05). Besides, there were no statistic differences (P>0 05) in sperm motility and fracture rate between one-step and multiple-step freezing methods. Conclusion: Cryopreservation induces the descent of sperm motility and leads to abnormal sperm characteristic which doesn't conspicuously result in the increase of incidence of sperm fracture.