A型肉毒杆菌毒素的基因测序与分析

对国内A型肉毒杆菌毒素基因测序，分析了解毒素基因结构并推测其氨基酸序列。提取肉毒杆菌总DNA，利用自行设计的引物对目的基因进行PCR扩增，把目的基因导入E.Coli JM109，筛选含目的基因的阳性克隆菌进行测序和分析，从而了解毒素基因结构并推测其氮基酸序列。对A型肉毒杆菌毒素基因的核苷酸序列成功测序并与GenBank中的相应序列比较，其序列同源性为96％。推测其氨基酸序列同源性为100％。成功地对A型肉毒杆菌毒素的基因进行了测序，这为肉毒杆菌毒素中毒的快速诊断，以及进一步探求以基因工程方法生产此毒素奠定基础。

Gene Sequence and Analysis of the Clostridium Botulinum Toxin of Type A

Aim: In order to learn nucleotide sequences of Clostridium botulinum neurotoxin type A(BoNT/A) and deduce its amino acid sequences, the authors analyzed the gene of the toxin .Methods: Firstly, extracting total DNA from Clostridium botulinum type A and then achieving the target gene by amplifying with a pair of specific primers designed by themselves. Secondly, transporting the target gene to Ecoli JM109. Finally, selecting positive E.Coli JM109 containing target gene and analyzing it. Results: Nucleotide sequences of the genes in the study showed 96% identity with that of BoNT/A from GenBank, but The deduced amino acid sequence of BoNT/A is completely identical. Conclusions: The authors have successfully detected gene sequence of botulinum neurotoxin type A and this will help researchers to produce the neurotoxin by gene engineering and make it possible to diagnose rapidly.