Localization of cholesterol in the Golgi apparatus of cardiac muscle cells

Summary Filipin (a polyene) interacts with cholesterol in membranes, producing distinctive deformations that can be detected by freeze-fracture. The distribution of filipin-induced deformations in the Golgi apparatus of cardiac myocytes suggests a role for this organelle in the transformation of cholesterol-poor membrane to cholesterol-rich membrane.Acknowledgments. This work was supported by a grant (No. 779) from the British Heart Foundation. I thank Dr E. Massey and Mr A. Slade for their help. Filipin was generously donated by Upjohn Ltd, Crawley, Sussex, U.K.