马尾松种源与内源生根抑制物的相关性

用浸渍、萃取和高效液相色谱法（HPLC）以同龄马尾松不同种源的同种源10个单株的插穗内源抑制物质（MIn)进行分离提纯。对MIn作初步定性和生物鉴定。用HPLC自动积分仪法比较MIn各组分含量。研究结果表明MIn都含7个组分, 其中4个组分属黄酮类,3个组分属苯酚类。种源间MI2、MI3、MI4和MI5差异显,MI1、MI6和MI7含量具显性差异。种源MI2与经度呈显正相关,MI4和MI5与纬度呈显负相关。江西吉安种源10个单株间MI4、MI6和MI7差异显MI1、MI2、MI3和MI5含量差异极显。MIn总相对含量以江西吉安为最高,广东韶关为最低。10个单株MIn总相对含量以3号母株为最低,9号母株为最高。MIn各组分抑制云南松种子发芽率的程度与其含量高低一致。MIn的存在是马发尾松插穗难发根的原因之一,且MIn含量高低是不同种源插穗生根能力变异的重要原因。阻遏控制MIn的基因表达,将是提高马尾松扦插成活率的主要措施之一。

A Study on Variation of the Endogenous Inhibitors in Pinus massoniana Cuttings

The endogenous inhibitors in Masson pine( Pinus massoniana Lamb.)cuttings(MI n )from the ortets of five different provenances and from ten ortets in Jiangxi provenance,were purified and separated by the methods of extraction and high pressure liquid chromatography(HPLC).The properties of the MI n were preliminarily proofed by means of chemical detection and ultraviolet absorption spectra followed by biological assay with seed germination percentage of Pinus yunnanensis Franch.Seven components were found in each cutting,four were flavones and three were phenols.The rooting rate of each kind of cuttings negatively correlated with their MI n contents.The contents of MI n in different cuttings were compared by HPLC auto integrator.The relative contents of MI 2,MI 3,MI 4 and MI 5 in cuttings from the five provenances were notably different.MI 1,MI 6 and MI 7 were very notably different.The total relative content of MI n in Jiangxi provenance was the highest,and the lowest in Guangdong provenance.The relative content of MI 2 was notably correlated with longitude,but MI 4 and MI 5 were negatively correlated with latitude.The relative contents of MI 4,MI 6 and MI 7 in ten ortets from Jiangxi provenance were notably different,and MI 1,MI 2,MI 3 and MI 5 were very notably different.MI n in No.3 ortet was the highest and No.9 the lowest.These all held an identical result with the cutting rooting test.The exist of MI n in cuttings was one of the reasons why Masson pine cuttings were difficult to root and why rootability of each kind of cutting was variant.Stopping the express of the MI n genes would be the successful way for increassing the cutting rooting rate.