| 共振光散射光谱法测定微量蛋白质 |
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| 引用本文： | 仲慧.共振光散射光谱法测定微量蛋白质[J].淮阴师范学院学报(自然科学版),2006,5(2):135-139. |
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| 作者姓名： | 仲慧 |
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| 作者单位： | 江苏省低维材料化学重点建设实验室,淮阴师范学院化学系,江苏,淮安,223300 |
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| 基金项目： | 江苏省教育厅自然科学基金;江苏省实验室基金 |
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| 摘 要： | 利用光吸收和共振光散射(RLS)光谱研究了铝试剂(ATA)与蛋白质在水溶液中的相互作用.在pH2.50时,蛋白质可使弱的ATA光散射信号曾强.基于这种现象,我们运用RLS技术,建立了测定纳克级蛋白质的方法.该方法简单、实用、灵敏.BSA的线性范围为0.010~27.4μg/mL,HSA的线性范围为0.010~30.5μg/mL.BSA的检测限为10.7 ng/mL,HSA的检测限为10.2 ng/mL.对实际人血清样品中的蛋白质进行了测定,其结果与临床方法一致.氨基酸、金属离子或其它共存化合物的干扰很小.
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| 关 键 词： | 共振光散射 铝试剂 蛋白质 测定 |
| 文章编号： | 1671-6876（2006）02-0135-05 |
| 收稿时间： | 2006-02-22 |
| 修稿时间： | 2006年2月22日 |
Microdetermination of Protein Using Resonance Light-scattering Method |
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| ZHONG Hui.Microdetermination of Protein Using Resonance Light-scattering Method[J].Journal of Huaiyin Teachers College(Natrual Science Edition),2006,5(2):135-139. |
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| Authors: | ZHONG Hui |
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| Abstract: | The interaction of the Indophenol Blue(ATA) with proteins in aqueous solution has been studied by optical absorption and Rayleigh Light Scattering(RLS) spectra.At pH?2.5,the weak RLS of ATA can be enhanced by the addition of proteins.Based on this,a novel method for the determination of proteins at nanogram levels by using RLS technique has been developed.Under optimum conditions,the linear range is 0.010～27.4?μg/mL for bovine serum albumin(BSA) and 0.010～30.5?μg/mL for human serum albumin(HSA).The method is simple,practical and much more sensitive,and it has been applied to the determination of proteins in human serum samples,the results are very close to those provided by clinical physicians.There is very little interference from amino acids,metal ions and other coexisting compounds. |
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| Keywords: | resonance light scattering aluminon protein determination |
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