| 电喷雾质谱研究谷胱甘肽与B族维生素的非共价复合物 |
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| 引用本文： | 储艳秋,蒋公羽,戴兆云,郑松柏,丁传凡. 电喷雾质谱研究谷胱甘肽与B族维生素的非共价复合物[J]. 复旦学报(自然科学版), 2009, 48(1) |
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| 作者姓名： | 储艳秋 蒋公羽 戴兆云 郑松柏 丁传凡 |
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| 作者单位： | 储艳秋,蒋公羽,丁传凡,CHU Yan-qiu,JIANG Gong-yu,DING Chuan-fan(复旦大学化学系激光化学研究所,上海,200433);戴兆云,郑松柏,DAI Zhao-yun,ZHENG Song-bai(复旦大学附属华东医院,上海,200040) |
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| 摘 要： | 为了研究谷胱甘肽与水溶性B族维生素的相互作用,采用电喷雾质谱结合紫外光谱,研究具有重要生理功能的谷胱甘肽与B族维生素形成的非共价复合物,B族维生素包括维生素B1(硫胺素),维生素B2(核黄素)和维生素B3(烟酰胺).非共价复合物的合成是将B族维生素和谷胱甘肽按一定化学计量比混合,然后在室温和pH 6～8条件下,将混合物在培养箱内培育60 min,使反应完全.电喷雾质谱表明谷胱甘肽可以与B族维生素形成非共价复合物.串级质谱中的碰撞诱导解离(CID)和紫外光谱进一步确认非共价复合物的生成.
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| 关 键 词： | 谷胱甘肽 B族维生素 非共价复合物 电喷雾质谱 |
Investigation on the Non-covalent Complexes of Glutathione and Vitamin B by Mass Spectrometry |
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| CHU Yan-qiu,JIANG Gong-yu,DAI Zhao-yun,ZHENG Song-bai,DING Chuan-fan. Investigation on the Non-covalent Complexes of Glutathione and Vitamin B by Mass Spectrometry[J]. Journal of Fudan University(Natural Science), 2009, 48(1) |
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| Authors: | CHU Yan-qiu JIANG Gong-yu DAI Zhao-yun ZHENG Song-bai DING Chuan-fan |
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| Affiliation: | 1.Laser Chemistry Institute;Chemistry Department;Fudan University;Shanghai 200433;China;2.Huadong Hospital Affiliated with Fudan University;Shanghai 200040;China |
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| Abstract: | To investigate the non-covalent interaction between glutathione and Vitamin B,a stoichiometry of reduced glutathione and three Vitamins,including Vitamin B1(thiamine),Vitamin B2(riboflavin),and Vitamin B3(nicotinamide) were mixed respectively,and then incubated at room temperature for 60min to reach the equilibrium.The electrospray ionization mass spectrometry(ESI-MS) results indicated that glutathione and three Vitamin B could form non-covalent complexes with all the three vitamins in physiological pH cond... |
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| Keywords: | glutathione vitamin B noncovalent complex electrospray ionigation mass spectrometry |
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