家蚕微孢子虫侵染家蚕胚胎细胞与草地贪夜蛾细胞的病变比较

家蚕微孢子虫(Nosema bombycis)是引起家蚕微粒子病的病原,是一种无线粒体的专性细胞内寄生的原虫.将N.bombycis经KOH处理后,接种于家蚕胚胎细胞(BmE细胞)和草地贪夜蛾卵巢细胞(Sf21).用倒置显微镜对微孢子虫在宿主细胞中感染增殖过程中的形态变化进行了跟踪观察,比较两种细胞接种N.bombycis后所出现的形态学变化.接种后第12天起,两种细胞内均充满不同发育阶段的孢子,并可见大量的胞外游动.在感染晚期,Sf21中有许多孢子从细胞中逸出,留下许多空泡,细胞还保持一定的完整性,而家蚕胚胎细胞则在感染后期完全崩解.这种差异可能是Sf21作为一种非原宿主细胞,对N.bombycis的感染有一定耐受性有关.

Comparison of Cytopathic Effect (CPE) of Sf21 Cell and Embryo Cell of Silkworm Infected by Nosema bombycis

Nosema bombycis is the pathogen of Pebrine of silkworm.It is an obligate intracellular parasite without mitochon-dria.After treatment with KOH,N.bombycis is inoculated into two cell lines,embryo cell of B.mori and Sf21 respectively.The comparison of morphological changes of the two cell lines Post Inoculation is conducted.The results show that after inoculation 12 days most of cells are filled with spores in different stages,and some spores are released from the cells.At the late stage of infection,many vacuoles with spore shape are observed in the Sf21 cell and membrane integrity is partially conserved.For BmE cell,the infected cells ware disrupted into debris.It may be that Sf21 is non-original host cell of N.bombycis.It can bear heavier burden of infection than BmE,the original cell.