| 应用光敏生物素探针检测杂交瘤及细胞中的小鼠白血病病毒 |
| |
| 引用本文： | 贺争鸣,刘佐民.应用光敏生物素探针检测杂交瘤及细胞中的小鼠白血病病毒[J].实验动物科学,1994,11(4):3-5. |
| |
| 作者姓名： | 贺争鸣 刘佐民 |
| |
| 作者单位： | 中国药品生物制品检定所 |
| |
| 摘 要： | 采用ＢａｍＨＩ酶切和琼脂糖凝胶电泳技术，从重组质粒ＰｓＦｌｌ中回收５ｋｂ小鼠白血病病毒（ＭｕＬＶ）ＤＮＡ片段，经光敏生物素标记后制备ＤＮＡ探针，以斑点杂交法检测杂交瘤细胞、ＭｃＡｂ纯品以及ＳＰ２／０细胞中的ＭｕＬｖ。结果表明：此探针灵敏度可达２５ｐｇ，特异性好，在被检测的１３株杂交瘤细胞和２株ＳＰ２／０细胞中分别查出５株和１株为ＭｕＬｖ阳性，ＭｃＡｂ纯品中未发现ＭｕＬｖ。
|
| 关 键 词： | 光敏生物素探针 小鼠 白血病病毒 杂交瘤细胞 |
Detection of MuLv in Hybridoma and other cell line with
Photobiotin-labeled Probe |
| |
| Hezhengming Liu Zuomin WeiLi,WuHuiying National Institute for the Control of Pharmaceutical and,Biological Products Beijing.Detection of MuLv in Hybridoma and other cell line with
Photobiotin-labeled Probe[J].Shiyan Dongwu Kexue,1994,11(4):3-5. |
| |
| Authors: | Hezhengming Liu Zuomin WeiLi WuHuiying National Institute for the Control of Pharmaceutical and Biological Products Beijing[ |
| |
| Institution: | Hezhengming Liu Zuomin WeiLi
WuHuiying National Institute for the Control of Pharmaceutical and
Biological Products Beijing[ |
| |
| Abstract: | MuLV DNA fragment(5kb)in
recombinant plasmid,Which wasdigested with restriction enzyme,was
isolated by agarose gel electrophoresis and labeled with photobiotin
as a probe. The probe was used to detect MuLv in hybridoma
cells,purified McAb products and SP2/C cells with dot
hyoridization.The result showed that the probe issensitive and
specific to detect MuLv under 25 pg homologous DNA. In total of l3
hybridoma and 2 SP2/O cells detected.the positive rate was 38%and
50%respectively. There was no MuLv DNA founded inp uri- fiedMcAb
products. |
| |
| Keywords: | Photobiotin-labeled probe MuLv Hybridoma Myeloma Purified McAb products |
| 本文献已被 CNKI 维普 等数据库收录！ |
