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| 重组毕氏酵母结构模型的改进及实验验证 | |
| 引用本文： | 任海涛,袁景淇,邓建慧,贺秉坤.重组毕氏酵母结构模型的改进及实验验证[J].上海交通大学学报,2004,38(4):551-554. |
| 作者姓名： | 任海涛 袁景淇 邓建慧 贺秉坤 |
| 作者单位： | 1. 上海交通大学,自动化系,上海,200030 2. 上海交通大学,自动化系,上海,200030;华东理工大学,生物反应器工程国家重点实验室,上海,200237 3. 华北制药集团新药中心,石家庄,050015 |
| 基金项目： | 国家自然科学基金项目(60174024)，国家高技术研究发展计划(863)资助项目(2001AA413110) |
| 摘 要： | 对早先建立的甲醇营养型重组毕氏醇母(Pichia pastoris)结构模型的产物生成部分进行了动态改进．甲醇流加初期蛋白生成所需的酶系尚处于诱导期，为了描述该酶系浓度和活性从低到高对产物比生成速率的影响，引入了一阶闭环调节器模型加以逼近。同时考虑了细胞体积增大对胞内酶系浓度的稀释作用．设计了6次实验以验证模型的有效性．结果表明，该模型能对细胞生长和蛋白生成给出满意的描述，同时。各罐批间参数变化不是很大，表明该模型具有较强的鲁棒性． |
| 关 键 词： | 毕氏酵母 重组人血清白蛋白 结构模型 模型验证 |
| 文章编号： | 1006-2467(2004)04-0551-04 |
| 修稿时间： | 2003年4月13日 |
Modification and Validation of the Structured Model for P.pastoris |
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| REN Hai-tao,YUAN Jing-qi.Modification and Validation of the Structured Model for P.pastoris[J].Journal of Shanghai Jiaotong University,2004,38(4):551-554. | |
| Authors: | REN Hai-tao YUAN Jing-qi |
| Institution: | REN Hai-tao~1,YUAN Jing-qi~ |
| Abstract: | The structured model proposed earlier for the recombinant Pichia pastoris was improved in the transients of product formation. At the beginning of the methanol growth phase, a first-order closed-loop regulator was introduced to describe the induction of the enzyme pool responsible for product formation. In this regulator model, the dilution effect resulted from cell growth on the enzyme pool was taken into account. Six cultivations were carried out for the purpose of model validation. The testing results indicate that the biomass and protein concentration can be well described by the improved model. In addition, relatively small deviation of the identified parameters among different experiments indicates the robustness of the model. |
| Keywords: | Pichia pastoris recombinant human serum albumin structure model model validation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |