石榴枯萎病菌生物学特性研究

石榴枯萎病（Pomegranate wilt）为甘薯长喙壳（Ceratocystis fimbriata Ellis＆Halsted）引起的一种真菌病害,是四川省攀西地区石榴上的一种新病害。对甘薯长喙壳菌的生物学特性研究结果表明：病原菌在供试甘薯葡萄糖琼脂（SPDA）、马铃薯甘薯葡萄糖琼脂、马铃薯葡萄糖琼脂（PDA）、玉米琼脂、石榴叶煎汁琼脂5种培养基中均能良好生长,而在查氏（Czapek）固体培养基和2%水琼脂培养基上不生长。能利用多种碳源,其中葡萄糖为最佳碳源。不同温度和pH对菌丝生长影响较大。菌丝在10-35℃范围内均能生长,最适温度为26℃,致死温度为50℃处理10min;菌丝生长的pH范围为3-6,最适pH为5。黑暗条件有利于病原菌菌丝的生长。

Research on the Biological Characteristics of Pomegranate Wilt

Pomegranate wilt is a fungal disease caused by Ceratocystis fimbriata,which was a new disease devastated in Sichuan Panzhihua-Xichang area.The biological characteristics of C.fimbriata were studied.The results showed that the mycelia could grow well in sweet potato dextrose agar（SPDA）,sweet potato and potato dextrose agar,potato dextrose agar（PDA）,corn meal agar and pomegranate leaf juice culture media,but never in the Czapek culture media or 2%water agar culture media.C.fimbriata could utilize carbon sources,and dextrose was the optimum.The mycelial growth was impacted by different temperature and pH.The temperature for mycelial growth ranged from 10℃ to 35℃,the optimum temperature was 26℃ and the lethal temperature of the mycelium was 50℃ for 10 minutes.The mycelia could grow from pH3 to pH6,and pH5 was the optimum.The dark was suitable for mycelium growth.