杀菌剂对木霉菌的毒性以及农业土壤中木霉菌分离与计数用选择性培养基

通过添加三唑酮刺激分生孢子产量、添加苯菌灵刺激营养生长以及添加曲里通X-100限制菌落扩展。本文进一步优化了木霉菌的选择性分离与计数培养基。三唑酮能有效地抑制轮枝菌生长。在该培养基上青霉菌和曲霉菌的生长受到明显抑制。在测定了多种杀菌剂对木霉菌以及相关的非木霉菌的毒性基础上，研制了更为可靠、有效的木霉菌选择性培养基。该培养基适合于从非木霉菌含量高的样本，也适合于从木霉菌含量低的样本中计数、分离木霉菌。无论样本中木霉菌的群体大还是小，利用该培养基均能进行计数和分离。

Toxicity of fungicides and selective medium development for isolation and enumeration of Trichoderma spp.in agricultural soils

A Trichoderma selective medium was modified further by adding Baytan to stimulate Trichoderma conidiation, benomyl to promote vegetative growth of Trichoderma, and Triton X-100 to limit the colony size. Baytan was effective in suppressing Verticillium spp. The modified medium inhibited Penicillium and Aspergillus growth. Toxicity of various fungicides to Trichoderma and associated non-Trichoderma fungi was investigated to assist the development of a more reliableTrichoderma selective medium. The new medium is suitable for quantitative isolation of Trichoderma spp. from samples with high counts of non-Trichoderma fungi or low counts of Trichoderma spp. The quantification and isolation of Trichoderma spp. from soils with both high and low populations is possible.