| 黄瓜Cd^2＋抗性的变化及其与蛋白质组分改变的关系 |
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| 引用本文： | 王艳,李雪梅,彭永康.黄瓜Cd^2＋抗性的变化及其与蛋白质组分改变的关系[J].天津师范大学学报(自然科学版),2008,28(1):14-18. |
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| 作者姓名： | 王艳 李雪梅 彭永康 |
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| 作者单位： | 1. 天津师范大学,化学与生命科学学院,天津,300387;天津商业大学,天津,300134
2. 天津师范大学,化学与生命科学学院,天津,300387 |
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| 基金项目： | 教育部科学技术研究重点项目 |
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| 摘 要： | 研究了在不同浓度Cd^2＋液处理下，黄瓜幼苗（cucumis sativus．L）蛋白质组分与含量的改变及其对于幼苗生长的影响．实验结果显示，随Cd^2＋处理浓度的增加（0．2～1．0mmol／L），黄瓜幼苗的根长、鲜重减少，叶绿素含量降低，但干重减少不明显．在1．0mmol／LCd^2＋溶液处理下，与对照相比，黄瓜幼苗的根、胚轴、叶绿体中的蛋白质组分发生变化，即蛋白缺失、新蛋白合成以及蛋白表达量的上调或下降，同时其生化性状（蛋白质组分、叶绿素含量）的变化与幼苗生长抑制呈正相关．
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| 关 键 词： | Cd^2+损害 2D-PAGE 叶绿体蛋白 黄瓜 |
| 文章编号： | 1671-1114（2008）01-0014-05 |
| 收稿时间： | 2007-05-01 |
| 修稿时间： | 2007年5月1日 |
Changes in cadmium tolerance and its relationship with the protein contents and compositions variation in cucumber seedlings |
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| WANG Yan,LI Xuemei,PENG Yongkang.Changes in cadmium tolerance and its relationship with the protein contents and compositions variation in cucumber seedlings[J].Journal of Tianjin Normal University(Natural Science Edition),2008,28(1):14-18. |
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| Authors: | WANG Yan LI Xuemei PENG Yongkang |
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| Institution: | WANG Yan , LI Xuemei , PENG Yongkang (1. College of Chemistry and Life Science, Tianjin Normal University, Tianjin 300387, China; 2. Tianjin University of Commerce, Tianjin 300134, China) |
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| Abstract: | The changes in protein contents, compositions and its effects on seedlings growth in cucumis sativus. L treated with Cd^2＋ solutions of different concentration were studied. The results showed that the root length, seedling height, fresh weight were decreased gradually in cucumber seedlings treated with Cd2＋ concentration ranging from 0. 2 to 1.0 mmol/L, and the change in dry weight wasrft obvious. The contents of chlorophyll were also decreased. New protein spots were induced and some protein spots were lost in root, plumular axis, chloroplast, when seedlings were treated with 1.0 mrnol/L Cd^2＋. A positive relationship was observed between the inhibition of seedling growth and changes in biochemical characteristics. |
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| Keywords: | cadmium damage 2D-PAGE chloroplast protein cucumis sativus L |
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