Effect of thrombin on the apoptosis of hippocampal neurons in vitro |
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Authors: | Yang Wen-qiong Sun Sheng-gang Tong E-tang and Gao Xue-bing |
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Institution: | (1) Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, Hubei, China |
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Abstract: | Hippocampal neurons were treated by thrombin and thrombin receptor activating peptides (TRAP). Cell survival rate was decreased
in a dose-dependent manner by MTT assay. The numbers of apoptotic cell and apoptotic rate of hippocampal neurons treated by
different concentrations of thrombin were increased in a dose-dependent manner by terminal deoxynucleotidyl transferase (TdT)
mediated dUTP-biotin nick end-labeling (TUNEL) method and Flow Cytometry. When the concentration of thrombin is 40 U/mL, TUNEL
positive cells and apoptotic rate of hippocampal neurons reached peak value, were 27.3±4.0 and (29.333±4.633)%, respectively.
Immunocytochemistry assay show that Bcl-2 protein expression was down-regulated and Bax protein expression was up-regulated
with the concentration of thrombin increased. TRAP can mimic the effect of thrombin to induce apoptosis on hippocampal neurons.
These data demonstrated that thrombin induced hippocampal neuron apoptosis in a dose-dependent manner through activating protease-activated
protein-1 (PAR-1). The change in expression of Bcl-2 and Bax was related with the effect of high concentration thrombin induced
apoptosis on hippocampal neurons.
Foundation item: Supported by the Natural Science Foundation of Hainan Province (N30215)
Biography: YANG Wen-qiong (1968-), female, Ph.D. candidate, research direction: cerebrovascular disease. |
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Keywords: | thrombin hippocampal neurons apoptosis protease-activated receptor-1 |
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