促进剂对木耳液体发酵生产的影响

【目的】有效地促进木耳菌体生长及胞外多糖的生产,确定可显著提高木耳液体发酵生产的促进剂及其添加量。【方法】在木耳液体发酵培养基中分别添加20种不同的促进剂,每种促进剂设置4个浓度,发酵6d后检测发酵液中的菌丝体、胞外多糖含量。然后对促进效果较好的Tween40、硬脂酸、棕榈酸、油酸进行正交试验。【结果】单因素试验结果表明,0.1%的棕榈酸、1%的硬脂酸、0.5%的Tween40、0.1%的油酸均可显著促进木耳胞外多糖的生产。正交试验结果表明,在发酵培养基中添加剂最佳组合为0.5%Tween40、1.5%硬脂酸、0.1%棕榈酸、0.25%油酸。【结论】硬脂酸、棕榈酸、油酸和Tween40可显著促进木耳胞外多糖的发酵生产。

Effects of Stimulators on the Liquid Fermentation of Auricularia auricula

Objective]To effectively enhance the mycelia growth and extracellular polysaccharide production of Auricularia auricula,stimulators for liquid fermentation of A.auricula were screened.Methods]Twenty stimulators were respectively added to the fermentation media at four different concentrations.The extracellular polysaccharide and mycelia biomass were determined after 6 d of fermentation.Moreover,Tween40,stearic acid,palmitic acid and oleic acid were selected for orthogonal experiment.Results]0.1% of palmitic acid,1% of stearic acid,0.5% of Tween40 and 0.1% of oleic acid in the culture medium significantly increased the yield of extracellular polysaccharide.Orthogonal experiment results showed that the optimal stimulator combination in the fermentation media was 0.5% Tween40,1.5% stearic acid,0.1% palmitic acid,0.25% oleic acid.Conclusion]Stearic acid,palmitic acid,oleic acid and Tween40 could promote the extracellular polysaccharide bio-synthesis in submerged culture of A.auricula.