Occurrence of 3-epi-22-deoxy-20-hydroxyecdysone and its phosphoric ester in diapause eggs of the silkworm,Bombyx mori |
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| Authors: | Y. Mamiya H. Sonobe K. Yoshida N. Hara Y. Fujimoto |
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| Affiliation: | (1) Department of Biology, Konan University, Higashinada-ku, 658 Kobe, Japan;(2) Department of Chemistry, Tokyo Institute of Technology, 152 Meguro-ku, Tokyo, Japan |
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| Abstract: | ![]()
Ecdysteroids in diapause eggs of the silkworm,Bombyx mori, were analyzed using high-performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). A relatively large amount of an unidentified free ecdysteroid and its phosphoric ester (conjugated form) were detected. These two compounds were isolated by a combination of column chromatography on silicic acid, thin-layer chromatography (TLC), and HPLC using a reverse-phase (RP) column. The purified compounds were identified as 3-epi-22-deoxy-20-hydroxyecdysone (22d20E ) and 3-epi-22-deoxy-20-hydroxyecdysone 2-phosphate (22d20E2P) by means of mass spectrometry and nuclear magnetic resonance spectroscopy. to our knowledge, this is the first report of 22d20E and 22d20E2P. |
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| Keywords: | Molting hormone 3-epi-ecdysteroids ecdysteroid conjugate silkworm Bombyx mori |
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