| 柔嫩艾美耳球虫地克珠利与马杜霉素耐药株的3种同工酶分析 |
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| 引用本文： | 郭涛, 董辉, 黄兵, 赵其平, 韩红玉, 姜连连, 李洋, 平宪卿, 朱顺海.柔嫩艾美耳球虫地克珠利与马杜霉素耐药株的3种同工酶分析[J].上海师范大学学报(自然科学版),2011,40(2):197-201. |
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| 作者姓名： | 郭涛 董辉 黄兵 赵其平 韩红玉 姜连连 李洋 平宪卿 朱顺海 |
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| 作者单位： | 1. 上海师范大学生命与环境科学学院,上海,200234
2. 中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室,上海,200241 |
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| 基金项目： | 国家自然科学基金项目(30700601) |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳对柔嫩艾美耳球虫(E imeria tenella)药物敏感株、地克珠利耐药株、马杜霉素耐药株以及2个耐药株重组体的乳酸脱氢酶(LDH)、磷酸葡萄糖异构酶(GPI)和葡萄糖-6-磷酸脱氢酶(G6PD)同工酶进行了分析.结果显示:在LDH同工酶谱中,敏感株有一条浓带,3株耐药株均没有酶带;在GPI同工酶谱中,敏感株有4条带,地克珠利耐药株有GPI-2和GPI-4,而马杜霉素耐药株和重组体均含有GPI-2、GPI-3和GPI-4;在G6PD同工酶谱中,敏感株有4条带,而3株耐药株均只有G6PD-3和G6PD-4.同工酶分析表明,药物敏感株与3株耐药株之间的3种同工酶均有差异.3株耐药株之间的GPI存在差异,而在LDH和G6PD无差异.
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| 关 键 词： | 柔嫩艾美耳球虫 地克珠利 马杜霉素 耐药性 同工酶 |
Three isozymes of eimeria tenella diclazuril-resistant strain and maduramycin-resistant strain |
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| GUO Tao,DONG Hui,HUANG Bing,ZHAO Qi-ping,HAN Hong-yu,JIANG Lian-lian,LI Yang,PING Xian-qing,ZHU Shun-hai.Three isozymes of eimeria tenella diclazuril-resistant strain and maduramycin-resistant strain[J].Journal of Shanghai Normal University(Natural Sciences),2011,40(2):197-201. |
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| Authors: | GUO Tao DONG Hui HUANG Bing ZHAO Qi-ping HAN Hong-yu JIANG Lian-lian LI Yang PING Xian-qing ZHU Shun-hai |
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| Institution: | GUO Tao1,DONG Hui2,HUANG Bing2,ZHAO Qi-ping2,HAN Hong-yu2,JIANG Lian-lian2,LI Yang2,PING Xian-qing2,ZHU Shun-hai2(1.College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China,2.Shanghai Veterinary Research Institute,CAAS / Key Laboratory for Animal Parasitology of Ministry of Agriculture,Shanghai 200241,China) |
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| Abstract: | The different isozyme spectrums of lactic dehydrogenase(LDH),glucose phosphoric acid isomerase(GPI) and glucose-6-phosphoric acid dehydrogenase(G6PD) among the Eimeria tenella sensitive strain,diclazuril-resistant strain,maduramycin-resistant strain and recombinant strain were analyzed by using the polyacrylamide gel electrophoresis.The results indicated that one band of LDH isozyme was present in the sensitive strain,while no band in 3 drug resistance strains.Four bands of GPI isozyme were characterized in... |
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| Keywords: | eimeria tenella diclazuril maduramycin drug resistance isozyme |
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