| TB过敏蛋白对小鼠体内几种酶活性的影响 |
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| 引用本文： | 李玉英,张政,白艳,王转花.TB过敏蛋白对小鼠体内几种酶活性的影响[J].山西大学学报(自然科学版),2007,30(1):95-97. |
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| 作者姓名： | 李玉英 张政 白艳 王转花 |
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| 作者单位： | 山西大学,化学生物学与分子工程教育部重点实验室,山西,太原,030006 |
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| 基金项目： | 国家自然科学基金;山西省青年科研项目;山西大学校科研和教改项目 |
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| 摘 要： | 研究了注射苦荞麦过敏蛋白TB前后小鼠体内的几种酶活性变化.以小鼠的心脏、肝脏、肾脏为实验材料,对其体内的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性及同工酶进行分析.测活结果显示:注射TB过敏蛋白后小鼠心脏和肝脏中SOD、POD、CAT的活性明显提高,而肾脏中几种酶活性均有所下降.电泳结果表明:SOD、POD的同工酶谱均发生了变化,谱带的酶活性不同程度的增加或减弱.
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| 关 键 词： | 苦荞麦 过敏蛋白 抗氧化酶 同工酶 小鼠 |
| 文章编号： | 0253-2395（2007）01-0095-03 |
| 修稿时间： | 2006-02-202006-05-09 |
Effect of Allergenic Protein from Tartary Buckwheat on Activity of Several Isoenzymes in Organs of Mice |
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| LI Yu-ying,ZHANG Zheng,BAI Yan,WANG Zhuan-hua.Effect of Allergenic Protein from Tartary Buckwheat on Activity of Several Isoenzymes in Organs of Mice[J].Journal of Shanxi University (Natural Science Edition),2007,30(1):95-97. |
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| Authors: | LI Yu-ying ZHANG Zheng BAI Yan WANG Zhuan-hua |
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| Institution: | Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Instittute of Bioteehnology ,Shanxi University, Taiyuan 030006,China |
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| Abstract: | The activity change of some enzymes in organs of mice injected with an allergenic protein from tartary buckwheat was studied.The activity of POD,SOD,CAT and the isoenzymes of POD,SOD in the heart,liver and kidney were analysed.The result showed that the allergenic protein had improved the activity of POD,SOD and CAT in the heart and liver of mice,and maybe because of the influence of the other proteins,however,the contrary result showed in the kidney of mice.The electrophoretic band spectrum of isoenzymes of POD,SOD including concentration and number also had a change.Some new bands appeared due to the improvement of activity of enzyme molecules,and contrarily,some bands disappeared due to the activity loss of enzyme molecules. |
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| Keywords: | tartary buckwheat allergenic protein antioxidant enzymes isoenzyme |
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