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克服香石竹组织培养中玻璃苗的研究
引用本文:赵艳岭,刘志强,邢红华,赵强,华金荣.克服香石竹组织培养中玻璃苗的研究[J].河南科学,2005,23(5):689-691.
作者姓名:赵艳岭  刘志强  邢红华  赵强  华金荣
作者单位:河南省科学院生物研究所,河南,郑州,450008;郑州中多电子计算机网络公司,河南,郑州,450000;河南省中医院,河南,郑州,450003
摘    要:香石竹试管苗玻璃化现象给试管苗的应用带来困难,采用强光照10000—20000 LX,在培养基中提高蔗糖和琼脂浓度,降低激素用量,对克服香石竹试管苗玻璃化有明显效果.青霉素对克服香石竹试管苗玻璃化无明显效果,但对生根有促进作用,其作用机制有待进一步研究.

关 键 词:香石竹  组织培养  玻璃苗  玻璃化现象
文章编号:1004-3918(2005)05-0689-03
收稿时间:2005-04-11
修稿时间:2005年4月11日

A study on decrease in vitrification shoots of carnation (Dianthus caryophyllus L. ) in tissue culture
ZHAO Yan-ling,LIU Zhi-qiang,XING Hong-hua,ZAO Qiang,HUA Jin-rong.A study on decrease in vitrification shoots of carnation (Dianthus caryophyllus L. ) in tissue culture[J].Henan Science,2005,23(5):689-691.
Authors:ZHAO Yan-ling  LIU Zhi-qiang  XING Hong-hua  ZAO Qiang  HUA Jin-rong
Abstract:The phenomenon of Vitrification phenomenon is a big obstruction in the propagantion of carnation by tissue culture, the effective way to decrease the vitrification phenomenon is to increase the concentration of sucrose and agar in the medium, to use strong luminosity of 10000-20000 LX, and to decrease the concentration of hormone. There is not evident effect of penicillin to control the vitrification shoots, but penicillin can stimulate the growth of carnation plantlet root in the medium, the mechanism of penicillin function to the root is not clear and it wll be studied further.
Keywords:carnation  tissue culture  vitrification phenomenon  vitrification shoots
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