| 6-BA与NAA不同浓度配比对大花蕙兰原球茎诱导的影响 |
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| 引用本文： | 章鹏程,陈瑜,邓衍福,陈芝娟,施农农.6-BA与NAA不同浓度配比对大花蕙兰原球茎诱导的影响[J].杭州师范大学学报(自然科学版),2012(4):331-336. |
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| 作者姓名： | 章鹏程 陈瑜 邓衍福 陈芝娟 施农农 |
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| 作者单位： | 杭州师范大学生命与环境科学学院植物RNA信号传导研究中心 |
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| 基金项目： | 国家自然科学基金项目(30770185);杭州市科技局重点实验室创新基金项目(20090230T05) |
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| 摘 要： | 以大花蕙兰茎尖为外植体,研究6-BA与NAA浓度梯度配比对大花蕙兰原球茎的诱导、增殖及分化的影响.结果表明:6-BA 1.0mg/L+NAA 0.5mg/L浓度配比下,大花蕙兰茎尖存活率达到93.3%;6-BA0.8mg/L+NAA 0.4mg/L浓度配比下,原球茎诱导率达到85.8%;6-BA 1.0mg/L+NAA 0.3mg/L浓度配比下,原球茎增殖系数达到4.59;6-BA 0.8mg/L+NAA 0.5mg/L浓度配比下,原球茎出芽率达到87.5%.首次报道采用6-BA 0.8mg/L+NAA 0.5mg/L浓度配比组合适合大花蕙兰茎尖培养,可同时获得较高的原球茎诱导率(80.8%)和出芽率(87.5%),比目前已报道的最佳原球茎诱导率(72.7%)和出芽率(76.7%)提高8%以上.
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| 关 键 词： | 大花蕙兰 茎尖 6-BA NAA 原球茎 |
Effects of 6-BA and NAA in Different Concentration Proportions on PLB Induction of Cymbidium Hybridum |
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| Authors: | ZHANG Peng-cheng CHEN Yu DENG Yan-fu CHEN Zhi-juan SHI Nong-nong |
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| Institution: | (Research Centre for Plant RNA Singnaling,College of Life and Environmental Sciences,Hangzhou Normal University, Hangzhou 310036,China) |
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| Abstract: | Shoot-tips from Cymbidium hybridum were used as the explants to investigate the effects of 6-BA and NAA in different concentration proportions on PLB(protocorm-like body) induction,propagation and differentiation.The results show that the survival rate of shoot-tips is 93.3% in the concentration proportion of 1.0 mg/L 6-BA + 0.5 mg/L NAA,the induction rate of PLB is 85.8% in the concentration proportion of 0.8 mg/L 6-BA + 0.4 mg/L NAA,the propagation rate of PLB is 4.59 in the concentration proportion of 1.0 mg/L 6-BA + 0.3 mg/L NAA,and the budding rate of PLB is 87.5% in the concentration proportion of 0.8 mg/L 6-BA + 0.5 mg/L NAA.The optimized concentration proportion of 0.8 mg/L 6-BA + 0.5 mg/L NAA can result in 8% higher ratio of PLB induction(80.8%) and differentiation(87.5%) than that with currently reported 72.7% and 76.7% respectively. |
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| Keywords: | Cymbidium hybridum shoot-tip 6-BA NAA protocorm-like body |
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