共培养技术及化学絮凝法对小球藻C.vulgaris XJB絮凝的影响

微藻是生物柴油理想的原料,但微藻尺寸较小,细胞密度较低,导致微藻采收困难。为提高微藻的采收率,节约成本,实验采用自絮凝斜生栅藻Scenedesmus sp.-BH与富油小球藻C.vulgaris XJB共培养技术来提高小球藻C.vulgaris XJB的絮凝率,同时与调节p H、添加Fe Cl3和添加壳聚糖3种化学絮凝法对比。结果表明:3种化学絮凝法60 min时的最佳絮凝率分别为84.2%、96.5%和72.9%;共培养最佳条件下小球藻C.vulgaris XJB 60 min时的絮凝率为33.3%,8 h后的絮凝率可达73.2%。共培养条件下小球藻C.vulgaris XJB的絮凝率比化学絮凝法的稍低,但该方法不会引入有害化学物质,后期分离成本低,是一种经济、环保的絮凝方法。

Effects of Co-culture and Chemical Flocculation Method on C.vulgaris XJB Flocculation

Microalgae are ideal raw materials of biodiesel production.However,their microscopic sizes and low cell density result in the difficulty of collection.In order to improve the recovery efficiency of microalgae and obtain a potentially efficient flocculation method,this work focused on co-cuhuring self-flocculating microalgae Scenedesmus sp.-BH and oil-rich microalgae C.vulgaris XJB.Moreover,adjusting pH,adding FeC13 or chitosan were tested in this work.Results showed that,the best flocculating rate for chemical floeculation method after 60 minutes were 84.2%,96.5% and 72.9%,respectively.The best flocculating rate of co-culture was 33.3% after 60 minutes,and the flocculating rate could reach 73.2% in 8 h.The C.vulgaris XJB＇s flocculating rate with co-culture method was lower than chemical flocculation method,but this method didn＇t introduce extra harmful chemicals into the product. It could be an economy and environmentally friendly flocculation method.