| 2,5—二氯硝基苯对脲酶活性的抑制及其动力学研究 |
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| 引用本文： | 马宝全,赵明.2,5—二氯硝基苯对脲酶活性的抑制及其动力学研究[J].南开大学学报,1997,30(4):107-108,112. |
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| 作者姓名： | 马宝全 赵明 |
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| 作者单位： | 南开大学生物化学及分子生物学系!天津，300071 |
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| 摘 要： | 本文应用动力学方法分析了２，５－二氯硝基苯对脲酶的抑制作用。实验结果表明，２，５－二氯硝基苯是尿酶的有效抑制剂，改变抑制剂和底物脲的浓度，测定脲酶活力并应用Ｌｉｎｅｗｅａｖｅｒ－Ｂｕｔｈ双倒数作图，测定该抑制剂对脲酶的抑制作用，结果均呈反竞争性抑制。
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| 关 键 词： | 二氯硝基苯 脲酶 抑制剂 活性 动力学 土壤 |
THE UREASE ACTIVITY INHIBITION OF 2, 5-DICHLORIDE MONONITRO-BENZENE AND A STUDY OF ITS KINETICS |
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| Ma Baoquan, Zhao Ming.THE UREASE ACTIVITY INHIBITION OF 2, 5-DICHLORIDE MONONITRO-BENZENE AND A STUDY OF ITS KINETICS[J].Acta Scientiarum Naturalium University Nankaiensis,1997,30(4):107-108,112. |
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| Authors: | Ma Baoquan Zhao Ming |
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| Abstract: | This paper discusses applying method of kinetcs analyzed the 2, 5-dichloride mononitro-benzene to urease activity inhibition. The result of experimentation have proved the 2, 5-dichloride mononitro--benzene is an effective activator. Under different concentration of inhibitor and change the concentration of substrate urea, the activity of urease was determined. The Lineweaver Burk method showed that the inhibition activity of this inhibitor is uncompetitive. |
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| Keywords: | 2 5-dichloride mononitro-bezene urease inhibitor |
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