微血管内皮细胞与肺动脉平滑肌细胞增殖的相互调节

研究常氧下微血管内皮细胞(MVEC)与肺动脉平滑肌细胞(PASMC)增殖的相互调节。先滤膜培养大鼠肺微血管内皮细胞(LMVEC)后，再与PASMC复合培养，采用流式细胞仪检测PASMC周期的变化。复合培养后，正常情况下使PASMC的G1期细胞数减少，S＋G2／M期细胞数增多，即促进细胞生长；使LMVEC田C的G1期细胞数增多，S＋G2／M期细胞数减少，即抑制细胞生长。可见，MVEC与PASMC复合培养后，常氧下促进PASMC细胞生长，抑制LMVEC的增生，存在相互作用。

Interaction on the Proliferation of Smooth Muscle Cells and the Microvascular Endothelial Cell

To explore the interaction on the proliferation of pulmonary arterial smooth muscle cells (PASMCs) and cocul-tured lung microvascular endothelial cells (LMVECs), the cell cycles of the PASMCs and LMVEC were analysed with flow cy-tometry. Compared with homotypic, the PASMCs at G1 phase decreased, but the cells at S + G2/M phase increased; and theLMVECs at G1 phase increased, but the cells at S + G2/M phase decreased. The PASMCs growth by cocultured was en-hanced; otherwise, the growth of the LMVECs was inhibited. There is the interaction between them.