| 重组毕赤酵母体系生成PlUGT1蛋白适宜条件研究 |
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| 引用本文： | 胡小祥,吕旻,周文灵,李玲.重组毕赤酵母体系生成PlUGT1蛋白适宜条件研究[J].华南师范大学学报(自然科学版),2011,0(3). |
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| 作者姓名： | 胡小祥 吕旻 周文灵 李玲 |
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| 作者单位： | 1.1.华南师范大学生命科学学院 |
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| 基金项目： | 广东省科技计划项目(2009B020301003) |
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| 摘 要： | 通过单因素试验分别研究pH值和温度对重组毕赤酵母GS115/pPIZA-PlUGT1生长以及甲醇浓度、pH值和温度对诱导表达PlUGT1蛋白的影响.结果表明，pH6.5和30 ℃是培养重组毕赤酵母GS115/pPIZA-PlUGT1最佳的生长条件，重组毕赤酵母GS115/pPIZA-PlUGT1诱导表达蛋白的适宜条件为甲醇体积分数0.5%、30 ℃和pH5.0，该条件下培养72 h，培养液中的蛋白质量浓度为0.721mg/mL.
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| 关 键 词： | 关键词:重组毕赤酵母 PlUGT1蛋白 生长条件 表达条件 |
| 收稿时间： | 2010-11-18 |
STUDIES ON APPRORIATE CONDITIONS FOR EXPRESSING PlUGT1 PROTEIN IN RECOMBINANT PICHIA PASTORIS SYSTEM |
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| HU Xiaoxiang,LV Min,ZHOU Wenling,LI Ling.STUDIES ON APPRORIATE CONDITIONS FOR EXPRESSING PlUGT1 PROTEIN IN RECOMBINANT PICHIA PASTORIS SYSTEM[J].Journal of South China Normal University(Natural Science Edition),2011,0(3). |
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| Authors: | HU Xiaoxiang LV Min ZHOU Wenling LI Ling |
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| Institution: | HU Xiaoxiang,LV Min,ZHOU Wenling,LI Ling(School of Life Science,South China Normal University,Guangdong Key Lab of Biotechnology for Plant Development,Guangzhou 510631,China) |
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| Abstract: | Based on the single factor experiment, the effects of pH values and temperatures on growth of GS115/pPIZɑA-PlUGT1, and the effects of methanol concentrations, pH values and temperature on PlUGT1 protein production were studied. The optimal growth conditions of GS115/pPIZɑA-PlUGT1 were as follows: pH 6.5 and 30℃. The optimal expression conditions of PlUGT1 protein were found to be 0.5% of methanol concentration, 30℃ and pH 5.0. In this expression conditions for 72 h culture, the protein concentration in cultural solution is 0.721 mg/mL. |
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| Keywords: | recombinant Pichia pastoris PlUGT1 protein growth condition expression |
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