转基因水稻纯系对褐飞虱的抗性研究

利用基因枪法将含潮霉素抗性基因、ＧＵＳ报告基因和雪花莲凝集基因的２个质粒ｐＷＲＧ１５１５和ｐＲＳＳＧＡ１共同转化粳稻品种鄂宜１０５的成熟胚诱导的愈伤组织,从轰击的１５２块愈伤组织中共再生出２６株独立转基因植株,ＰＣＲ／Ｓｏｕｔｈｅｒｎ印迹法分析发现,７３％的转基因植株含有所有３个外源基因,遗传分析证实外泊基因在转基因植株后代中以孟德尔方式遗传,从其Ｒ１代亲本为孟德尔３：１方式遗传的Ｒ２代中,鉴定出

Studies on Resistance to Brown Planthopper in Transgenic Rice Pure Lines

Mature seed-derived callus from japonica rice cv. Eyi 105 was co-transformed with two plasmids,pWRG1515 and pRSSGNA1,containing the selectable marker hygromycin phosphotransferase gene (hpt),the reporter β-glucuronidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment.Twenty-six independent transgenic rice plants were regenerated from 152 bombarded callus. Seventy-three percent of transgenic plants contained all the three genes, revealed by PCR/Southen blot analysis.Genetic analysis confirmed Mendelian segregation of transgenes in progeny. From R2 generations with their R1 parent plants showing 3∶1 Mendelian segregation patterns, three independent homozyous lines expressing all the three transgenes were identified. Insect bioassay results showed that these homozygous lines had significant inhibition to brown planthopper.