| 阿维拉霉素高产菌株的推理选育 |
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| 引用本文： | 朱传合,贺亚男,杜连祥,路福平.阿维拉霉素高产菌株的推理选育[J].华南理工大学学报(自然科学版),2007,35(4):100-104,116. |
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| 作者姓名： | 朱传合 贺亚男 杜连祥 路福平 |
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| 作者单位： | 1. 山东农业大学,食品科学与工程学院,山东,泰安,271018
2. 天津科技大学,生物工程学院,天津,300222 |
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| 基金项目： | 天津市科技攻关重点专项(05YFGZGX04400) |
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| 摘 要： | 为获得阿维拉霉素高产菌株,在研究菌株Streptomyces viridocbromogenes96(SV-96)葡萄糖及氨盐代谢的基础上,采用“NTG随机诱变 氨基乙酸和2-脱氧-D葡萄糖联合抗性”的试验方案进行阿维拉霉素高产菌株的推理选育,获得了氨基乙酸和2-脱氧-D-葡萄糖联合抗性高产突变株SV-GT-596.该菌株遗传性能良好,阿维拉霉素产量高达56.4mg/L,较出发菌株提高了162.3%.实验表明,氨基乙酸和2-脱氧-D-葡萄糖抗性推理选育可克服随机筛选的盲目性,提高筛选工作效率.
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| 关 键 词： | 阿维拉霉素 推理选育 氨基乙酸 2-脱氧-D-葡萄糖 绿色产色链霉菌 |
| 文章编号： | 1000-565X（2007）04-0100-05 |
| 修稿时间： | 2006-03-24 |
Rational Screening of High-Yield Avilamycin-Producing Strain |
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| Zhu Chuan-he,He Ya-nan,Du Lian-xiang,Lu Fu-ping.Rational Screening of High-Yield Avilamycin-Producing Strain[J].Journal of South China University of Technology(Natural Science Edition),2007,35(4):100-104,116. |
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| Authors: | Zhu Chuan-he He Ya-nan Du Lian-xiang Lu Fu-ping |
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| Institution: | 1. College of Food Science and Engineering, Shandong Agricultural Univ. , Taian 271018, Shandong, China; 2. College of Biotechnology, Tianjin Univ. of Science and Tech. , Tianjin 300222, China |
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| Abstract: | In order to screen high-yield avilamycin-producing strain,a scheme of "random NTG mutation glycine and 2-deoxy-D-glucose resistant rational screening" was proposed after investigating the metabolism of glucose and ammonium by Streptomyces viridocbromogenes 96(SV-96).A glycine and 2-deoxy-D-glucose resistant mutant(SV-GT-596) with high yield was then obtained.It is found that SV-GT-596 strain is of good transmissibility,and that,by using the strain,the yield of avilamycin reaches 56.4 mg/L,which is 62.3% more than the initial yield by flake fermentation.Experimental results also indicate that the rational screening overcomes the blindness of random screening and improves the screening efficiency. |
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| Keywords: | avilamycin rational screening glycine 2-deoxy-D-glucose Streptomyces viridocbromogenes |
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