复合酶法提取褐藻可溶性膳食纤维的工艺优化及其抗氧化功能测定

以褐藻为原材料， 利用复合酶提取法提取可溶性膳食纤维(SDF). 先通过单因素实验研究不同提取条件对褐藻SDF提取率的影响， 再进行响应面实验分析， 从而确定复合酶法提取褐藻SDF的最佳条件； 测定所得褐藻 SDF 的理化特性， 并测定其抗氧化活性. 结果表明:  最佳提取条件为m(固)∶V（液）=1∶25, 55 ℃的酶解温度、 75 min的酶解时间、2.2%的酶添加量， 该条件下得到褐藻SDF的最大提取率为38.15%，相对偏差为2.23%；褐藻 SDF 的持水力和膨胀力分别为 24.6 g/g， 53.7 mL/g； 对1,1-二苯基-2-三硝基苯肼（DPPH.）自由基清除率达75.77%， 对2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS.）自由基清除率在褐藻SDF质量比为160 μg/g时与对照组无显著性差异.  

Optimization of Extraction Process of Soluble Dietary Fiber from Brown Algae by Complex Enzyme Method andDetermination of Its Antioxidant Function#br#

The soluble dietary fiber (SDF) was extracted from brown algae by complex enzyme extraction. Firstly, the effects of different extraction conditions on the extraction rate of brown algae SDF were studied  by single-factor experiment,  and then the  response surface analysis was carried out to determine  the optimal conditions for enzyme extraction of brown algae SDF,  and  the physicochemical properties of obtained brown algae  SDF and its antioxidant activity were determined. The results show that  the optimal extraction conditions are  m(solid)∶V（liquid）=1∶25, enzymatic hydrolysis temperature of  55 ℃, enzymatic hydrolysis time of 75 min and 2.2% enzyme addition, the maximum  extraction rate of brown algae SDF is  38.15%, and the relative deviation is 2.23%. The water holding capacity and swelling capacity  of brown algae SDF are 24.6 g/g and 53.7 mL/g respectively,  1,1-diphenyl-2-picrylhydrazyl  radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl（DPPH.） radical clearance rate is  75.77%, and there is no significant difference between the control group and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid）（ABTS.） radical clearance rate when brown algae SDF mass ratio is 160 μg/g.