迎春花无菌体系的建立以及茎段和叶片愈伤组织的诱导

本文对迎春花无菌体系的建立和茎段、叶片愈伤组织的诱导进行了研究。结果表明：（1）在建立迎春花的无菌体系时,应该选择晴天去采集外植体为好;（2）带芽茎段和叶片的污染率最大,而不带芽茎段的污染率最小;（3）在超净工作台外消毒应先用饱和洗衣粉溶液浸泡10 min,然后用自来水冲洗2-3次,最后再用蒸馏水冲洗2-3次;在进行超净工作台内消毒时,叶片应该先用84消毒液（1%）浸泡5min,无菌水冲洗3次,75%酒精浸泡20s,无菌水冲洗3次,0.1%升汞浸泡8 min,无菌水冲洗3次,而茎段应该先用84消毒液（1%）浸泡10 min,无菌水冲洗3次,75%酒精浸泡30s,无菌水冲洗3次,0.1%升汞浸泡10min,无菌水冲洗3次;（4）在NAA为0.2 mg/L时,高浓度的6-BA有利于不定根的再生,而低浓度的6-BA则更利于愈伤组织的诱导;在NAA为0.2 mg/L时,高浓度的KT有利于愈伤组织的诱导,而低浓度的KT则有利于不定根的再生;（5）NAA更利于不定根诱导,也可诱导愈伤组织。而2,4-D只利于愈伤组织的诱导,不利于诱导不定根。

The Establishment of Jasminum nudiflorum Lindl Aseptic System and Induction of Stem and Leaf Callus

This article studied the establishment of Jasminum nudiflorum Lindl aseptic system and the induction of Stem and leaf Callus. The result indicated that： （ 1 ） when estabhshing Jasminum nudiflorum Lindl aseptic system, explants gathering should be chose in the cloudless day; （2） The pollution rate of the stems with a bud and the leaves was largest, but the pollution rate of the sterns without a bud was smallest; （3） the disinfection should first use the saturated laundry powder solution to soak 10 minutes outside the ultra - clean table, then flushes 2 - 3 time with the running water, finally uses the distilled water to flush again 2 - 3 time; When carrying on to disinfect in the ultraclean table, the leaves should first use 84 disinfection hquid （ 1% ） to soak 5 minutes, the aseptic water flushes 3 times, 75% ethyl alcohol soak 20 seconds, the aseptic water flushes 3 times, 0.1% mercuric chloride soak 8 minutes, the aseptic water flushes 3 times, but for the stems, they should first use 84 disinfection hquid （ 1% ） to soak 10 minutes, the aseptic water flushes 3 times, 75% ethyl alcohol soak 30 seconds, the aseptic water flushes 3 times, 0.1% mercuric chloride soak 10 minutes, the aseptic water flushes 3 times; （4） when NAA is 0.2mg/L, high density 6 - BA was advantageous to adventitious mot regeneration, but low density 6 - BA then favored callus induction; （4） when NAA is 0.2 mg/L, high density KT is advantageous to callus induction, but low density KT then was advantageous to adventitious mot regeneration; （5） NAA favored adventitious mot induction, also may induced callus. But 2,4 - D only favored callus induction, which is disadvantageous to the adventitious mot induction.