| 金钗石斛兰组培苗的生根培养和移栽 |
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| 引用本文： | 张凤银,张萍,张静,杨波.金钗石斛兰组培苗的生根培养和移栽[J].江汉大学学报(自然科学版),2009,37(2):98-101. |
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| 作者姓名： | 张凤银 张萍 张静 杨波 |
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| 作者单位： | 1. 江汉大学,生命科学学院,武汉,430056
2. 中国科学院武汉植物园,武汉,430074 |
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| 摘 要： | 以1/2MS为基本培养基,添加不同浓度的NAA、IBA和6-BA,配置16种培养基进行试验,筛选最适宜于金钗石斛兰生根的培养基.结果显示:1/2MS+0.2 mg/L 6-BA+1.0 mg/L NAA+0.5 mg/L IBA及1/2MS+0.5 mg/L 6-BA+1.0 mg/L NAA+0.2 mg/L IBA培养基最适宜于金钗石斛兰生根,生根率达100%,而且根系生长健壮.将金钗石斛兰生根组培苗移栽于6种不同的基质中,结果表明,以棕榈外壳作为移栽基质最佳,移栽成活率达100%,且幼苗生长良好.
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| 关 键 词： | 金钗石斛 正交设计试验 培养基 激素 生根 移栽 |
Root Culture and Transplant of Dendrobium nobile Tissue Culture Seedling |
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| ZHANG Feng-yin,ZHANG Ping,ZHANG Jing,YANG Bo.Root Culture and Transplant of Dendrobium nobile Tissue Culture Seedling[J].Journal of Jianghan University:Natural Sciences,2009,37(2):98-101. |
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| Authors: | ZHANG Feng-yin ZHANG Ping ZHANG Jing YANG Bo |
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| Abstract: | Choosed 1/2 MS as the basic culture medium,plused NAA、IBA and 6-BA of dif-ferent concentrations,according to the L16(45) orthogonal experimental design,selected 16 culturemedia for experiment to filter the best culture medium for the rooting of Dendrobium nobile.Theresult indicated that 1/2 MS + 0.2 mg/L 6-BA+1.0mg/L NAA + 0.5 mg/L IBA and 1/2 MS + 0.5 mg/L6-BA + 1.0 mg/L NAA + 0.2 mg/L IBA were the best two for the rooting of Dendrobium nobile,asthe rooting rate was 100% and the root grew robustly.Transplanted Dendrobium nobile tissue cultureseedling to 6 substrates,the result indicated that the best substrate for transplanting was palm fibre,rate of survival was 100% and the seedling grew well. |
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| Keywords: | Dendrobium nobile orthogonal experimental design culture medium hormone rooting transplant |
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