孕期锰染毒对仔鼠脑、肝AchE的影响

为探讨孕期锰染毒对仔鼠脑、肝乙酰胆碱脂酶(ACHE)的影响,将妊娠大鼠随机分为3组,腹腔注射氯化锰溶液,待其自然分娩后,分别取1d,11d仔鼠脑、肝组织进行匀浆,检测匀浆AchE活性。结果表明,随着染锰剂量的增加,1d龄仔鼠肝AchE活性各组,下降趋势明显(P<0.05);但两染毒组之间差异无显著意义(P>0.05)。11d仔鼠脑、肝AchE活性对照组均显著高于染毒组。表明孕期锰染毒能抑制仔鼠肝、脑组织AchE活性,从而影响子代学习、记忆能力和神经肌肉接点处的兴奋传导。

Effect of Pregnant Rat's Exposure to Manganese on Acetylcholinesterase of Offspring's Brain and Liver

To study the effect of manganese on acetylcholinesterase(ACHE)in the filial rat's brain and liver after the mother-rat is exposed to manganese,pregnant rats are randomly divided into three groups and receive manganese chloride of different quantities by intraperitoneal injection for 8 times every other day. After normal delivery,the brains and livers of filial rats which are aged 1 day and 11 days are made into homogenate respectively and ACHE is measured.The results show that the decreasing trend of l-day-old filial rat brain ACHE is significant among the three groups(P<0.05). And while the decreasing trend of l-day-old filial rat liver ACHE is significant between control group and the two poisoned groups,the difference between the two poisoned groups is not significant(P>0.05).It is also found that,while the decreasing trend of 11-day-old filial rat brain and liver ACHE is significant between control group and high-dosage group, between low-dosage group and high-dosage group(P<0.05),the difference is not significant between control group and low-dosage group(P>0.05). It is therefore concluded that pregnant rats exposed to manganese can inhibit the activity of acetylcholinesterase in filial rat's brain and liver,and as a result the filial rat's ability of learning, remembrance and exciting conduction of nerve-muscle joint are influenced.