维生素A缺乏对大鼠生长发育及铁吸收的影响

目的研究维生素A(VA)缺乏对生长期大鼠生长发育及铁吸收的影响.方法将52只雄性SD大鼠,21日龄,按体质量随机分为4组,每组13只,Fe及VA正常对照组(Ⅰ组),Fe正常VA完全缺乏组(Ⅱ组),Fe正常VA轻度缺乏组(Ⅲ组),Fe和VA轻度缺乏组(IV组).实验动物喂饲8周,实验期末,进行72 h代谢实验,记录大鼠的进食量,并收集粪便.代谢实验结束后,处死大鼠,测定血清VA、粪铁含量,计算铁表观吸收率.结果各组动物除Ⅱ组动物性情暴躁、易激惹外,一般状况良好,维生素A缺乏可以使大鼠铁表观吸收率下降(P<0.05).结论维生素A缺乏可能通过影响铁吸收来影响体内铁的营养状况.

Effects of Vitamin A Deficiency on the Growth and Development and Iron Absorption in Rats

Objective To study effects of vitamin A(VA) deficiency on the growth and iron intake in rats.Method Fifty two male SD rats aging 21 days were divided randomly into four groups according to their body weights and each group included 13 rats.They were normal VA and iron group(Ⅰ),normal iron but complete lack VA group(Ⅱ),normal iron but slight deficiency VA group(Ⅲ) and slight VA and iron deficiency group(Ⅳ).The animals were raised for eight weeks.At the end of experiment,72 hours metabolic experiment was conducted in which the food intake of the rats was recorded and the feces were collected.After the metabolic experiment,the rats were sacrificed,the serum VA and the iron content in the feces were determined,and the apparent iron absorption rate was calculated.Results The results showed that animals in each group were generally in a good condition except the animals in group Ⅱ who were fiery and irritated,and vitamin A deficiency could induce a decrease in the iron apparent absorption rate(P<0.05).Conclusion VA deficiency may affect iron nutritional status by influencing the iron absorption.