| 新试剂N-(4-乙氧苯基)-N′-(4-安替吡啉基)硫脲与蛋白质相互作用的机理研究 |
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| 引用本文： | 崔凤灵,崔延瑞,卢雁,樊静.新试剂N-(4-乙氧苯基)-N′-(4-安替吡啉基)硫脲与蛋白质相互作用的机理研究[J].河南师范大学学报(自然科学版),2005(3). |
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| 作者姓名： | 崔凤灵 崔延瑞 卢雁 樊静 |
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| 作者单位： | 河南师范大学化学与环境科学学院 河南新乡453007
(崔凤灵，崔延瑞，卢雁)，河南师范大学化学与环境科学学院 河南新乡453007(樊静) |
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| 基金项目： | 国家自然科学基金资助项目(20443002) |
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| 摘 要： | 利用荧光光谱法研究了N-(4-乙氧苯基)-N′-(4-安替吡啉基)硫脲与血清白蛋白的作用机理,根据荧光猝灭效应求得了硫脲与血清白蛋白的结合常数,根据荧光非辐射能量转移理论和热力学参数确定了它们之间的主要作用力类型为疏水作用力.
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| 关 键 词： | N-(4-乙氧苯基)-N′-(4-安替吡啉基)硫脲(EPAT) 牛血清白蛋白 荧光光谱法 相互作用 |
A Study on the Reaction Between N-(4-ethoxyphenyl)-N'-(4-antipyrinyl) Thiourea and Bovine Serum Albumin |
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| CUI Feng-ling,CUI Yan-rui,LU Yan,FAN Jing.A Study on the Reaction Between N-(4-ethoxyphenyl)-N'-(4-antipyrinyl) Thiourea and Bovine Serum Albumin[J].Journal of Henan Normal University(Natural Science),2005(3). |
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| Authors: | CUI Feng-ling CUI Yan-rui LU Yan FAN Jing |
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| Abstract: | The study of the reaction between N-undecyl-N'(Sodiump- aminbenzensulfonate) thiourea (EPAT) and bovine serum albumin was reported using fluorescence quenching method and energy transfer technique. The binding constants were obtained. According to the thermodynamic parameters, the main sort of binding force can be known. The mechanism of quenching belongs to dynamic quenching and the main sort of binding force is mainly hydrophobic. |
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| Keywords: | N-(4-ethoxyphenyl)-N'-(4-antipyrinyl) thiourea (EPAT) bovine serum albumin fluorescence spectroscopy interaction |
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