| 利用DNA微卫星标记定位水稻的抗稻瘟病基因 |
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| 引用本文： | 刘士平,薛艳红.利用DNA微卫星标记定位水稻的抗稻瘟病基因[J].三峡大学学报(自然科学版),2003,25(6):574-576. |
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| 作者姓名： | 刘士平 薛艳红 |
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| 作者单位： | 三峡大学,化学与生命科学学院,湖北,宜昌,443002 |
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| 摘 要： | 利用回交育种中产生的回交群体结合前人的研究结果构建了Pil基因区域的局部分子标记连锁图，通过BC1F2家系的接种结果判断其基因型，将Pil定位在RFLP标记RZ536与SSR标记RM144之间，图距分别为9．7、6．8cm，从而建立了一套完整的以PCR为基础的分子标记辅助选择体系。
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| 关 键 词： | 微卫星标记 稻瘟病 基因定位 DNA |
| 文章编号： | 1007-7081(2003)06-0574-03 |
| 修稿时间： | 2003年5月13日 |
Locating Rice Blast Resistance Gene by DNA Microsatellite Markers |
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| Liu Shiping Xue Yanhong.Locating Rice Blast Resistance Gene by DNA Microsatellite Markers[J].Journal of China Three Gorges University(Natural Sciences),2003,25(6):574-576. |
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| Authors: | Liu Shiping Xue Yanhong |
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| Abstract: | The rice blast resistance gene Pi1 was located with a backcross population deriving from backcross breeding process. According to the inoculation results,F1829 was chosen to be the specific isolate to identify Pi1 gene. In the process of backcross breeding,a medial BC population came into being, by which a linkage map of Pi1 gene region was constructed. BC1F2 families were inoculated to identify the genotype. The gene was mapped on the top of chromosome 11 between markers RZ536 and RM144, with a distance of 9. 7 cm and 6. 8 cm respectively. Except some RFLP markers, two SSR markers were merged in the map, so a MAS system based on PCR markers was established. |
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| Keywords: | microsatellite marker rice blast disease gene tagging |
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